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IGF-II R/IGF2R Antibody

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Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.
Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of Hela cells (right) compared to ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Pl, PmSpecies Glossary
Applications WB, DB, ICC/IF, NULL
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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IGF-II R/IGF2R Antibody Summary

Immunogen
300 kDa cation-independent receptor purified from adult bovine liver tissue.
Marker
Late Endosome Marker
Specificity
CI-MPR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF2R
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Dot Blot 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Tissue Culture Substratum
  • Western Blot 1:2000 - 1:3000
Application Notes
In WB: Detects a 300 kDa protein representing Cl-MPR. May be useful in IP.
Publications
Read Publication using
NB100-74387 in the following applications:

  • WB
    1 publication

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26053072).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for IGF-II R/IGF2R Antibody

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

The Cation independent Mannose 6 Phosphate Receptor transports phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose 6 phosphate receptors in the Golgi apparatus and the resulting receptor ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-74387
IGF-II R/IGF2R Antibody
Species: Hu, Mu, Bv, Pl, Pm
Applications: WB, DB, ICC/IF, NULL

Publications for IGF-II R/IGF2R Antibody (NB100-74387)(1)

We have publications tested in 1 confirmed species: Plant.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-II R/IGF2R Antibody (NB100-74387). (Showing 1 - 1 of 1 FAQ).

  1. What is your modified RIPA buffer recipe?
    • 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGF2R
Entrez
Uniprot