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IGF-I R/IGF1R Antibody - BSA Free

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 (1 Review)
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Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.
Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.
Immunocytochemistry/ Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody ...read more
Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Eq, Pm, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.09 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77680AF350
Alexa Fluor 405
NBP1-77680AF405
Alexa Fluor 488
NBP1-77680AF488
Alexa Fluor 532
NBP1-77680AF532
Alexa Fluor 594
NBP1-77680AF594
Alexa Fluor 647
NBP1-77680AF647
Alexa Fluor 700
NBP1-77680AF700
Alexa Fluor 750
NBP1-77680AF750
Allophycocyanin
NBP1-77680APC
Biotin
NBP1-77680B
CoraFluor 1
NBP1-77680CL1
DyLight 350
NBP1-77680UV
DyLight 405
NBP1-77680V
DyLight 488
NBP1-77680G
DyLight 550
NBP1-77680R
DyLight 594
NBP1-77680DL594
DyLight 650
NBP1-77680C
DyLight 680
NBP1-77680FR
DyLight 755
NBP1-77680IR
FITC
NBP1-77680F
HRP
NBP1-77680H
Janelia Fluor 525
NBP1-77680JF525
Janelia Fluor 549
NBP1-77680JF549
Janelia Fluor 585
NBP1-77680JF585
Janelia Fluor 635
NBP1-77680JF635
Janelia Fluor 646
NBP1-77680JF646
Janelia Fluor 669
NBP1-77680JF669
PE
NBP1-77680PE
PerCP
NBP1-77680PCP
mFluor Violet 450 SE
NBP1-77680MFV450
mFluor Violet 500 SE
NBP1-77680MFV500
mFluor Violet 610 SE
NBP1-77680MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77680SS
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IGF-I R/IGF1R Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human IGF1 Receptor protein (between residues 700-800) [UniProt P08069]
Localization
Membrane.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1 ug/ml
Application Notes
This IGFR1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~100 kDa.
Control Peptide
IGF-I R/IGF1R Protein (NBP1-77680PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77680 in the following applications:
Publications
Read Publications using
NBP1-77680 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reactivity with Equine reported via customer feedback. Predicted to react with Bovine (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGF-I R/IGF1R Antibody - BSA Free

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(49 Publications)
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IRS1 Antibody
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Immunohistochemistry-Paraffin IRS1 Antibody
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Immunocytochemistry/ Immunofluorescence IRS1 Antibody
(4 Publications)
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EGFR Antibody
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AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
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IGFBP-3 [HRP]
DGB300
N/A IGFBP-3 [HRP]
N/A IGFBP-3 [HRP]
(47 Publications)
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Applications: ELISA
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Growth Hormone
Antibody [Unconjugate
AF1067
Western Blot Growth Hormone Antibody [Unconjugated]
ELISA Growth Hormone Antibody [Unconjugated]
Neutralization Growth Hormone Antibody [Unconjugated]
(15 Publications)
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Growth Hormone
Antibody [Unconjugate
AF1067
Western Blot Growth Hormone Antibody [Unconjugated]
ELISA Growth Hormone Antibody [Unconjugated]
Neutralization Growth Hormone Antibody [Unconjugated]
(15 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
IGFBP-1 [Biotin]
DY871
N/A IGFBP-1 [Biotin]
(16 Publications)
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Applications: ELISA
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236-EG
SDS-PAGE EGF [Unconjugated]
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Cell Culture EGF [Unconjugated]
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NBP1-90927
Western Blot gamma-glutamyl hydrolase Antibody
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Immunohistochemistry-Paraffin gamma-glutamyl hydrolase Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
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Immunocytochemistry/ Immunofluorescence IGF-II R/IGF2R Antibody (2G11) - BSA Free
(12 Publications)
Species: Bv, Hu, Mu, Pm, Rt
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
IGFBP-5 Antibody
AF578
Immunohistochemistry IGFBP-5 Antibody
Western Blot IGFBP-5 Antibody
Western Blot IGFBP-5 Antibody
(7 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB

Publications for IGF-I R/IGF1R Antibody (NBP1-77680)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
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Review for IGF-I R/IGF1R Antibody (NBP1-77680) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-77680:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot IGF-I R/IGF1R NBP1-77680
Enlarge 		4
reviewed by:
Christopher England
 WB Other 02/04/2016
View

Summary

ApplicationWestern Blot
Sample TestedHuman pancreatic cancer cell lines MiaPaCa-2, AsPC-1, and BxPC-3
SpeciesOther
LotA-1

Product General Protocols

View specific protocols for IGF-I R/IGF1R Antibody (NBP1-77680): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-I R/IGF1R Antibody (NBP1-77680). (Showing 1 - 1 of 1 FAQs).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

  ICC/IF Protocol
  IHC-P Protocol
  Western Blot Protocol
  SDS
  PDF Datasheet
  Request CofA

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Recent Reviews

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1
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Western Blot IGF-I R/IGF1R NBP1-77680
Enlarge
 Christopher England
02/04/2016
Application: WB
Species: Other
read this review

Bioinformatics

Gene Symbol IGF1R
Entrez
Uniprot