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IgE Antibody (RM122) - Azide and BSA Free

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Sandwich ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - Sandwich ELISA using RM122 as the capture antibody (25ng/well), and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase ...read more
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary ...read more
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - ELISA of human immunoglobulins shows RM122 reacts to both IgE from human myeloma and IgE from human plasma. No cross reactivity with human IgG, IgM, IgD, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ELISA
Clone
RM122
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Catalog# & Conjugate Size Price
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IgE Antibody (RM122) - Azide and BSA Free Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human IgE
Specificity
This antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IGHE
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA Capture 100 ng/well
  • Sandwich ELISA Detection 0.01 - 0.1 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IgE Antibody (RM122) - Azide and BSA Free

  • IgE
  • immunoglobulin heavy constant epsilon

Background

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for IgE Antibody (NBP3-25973)

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Blogs on IgE.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHE