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ICAM-1/CD54 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1:1000 µg/mL of Goat Anti-Human ICAM‑1/CD54 Polyclonal Antibody ...read more
ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human ICAM-1/CD54 Polyclonal Antibody (Catalog # BBA17) at 1:600 dilution for 3 hours at room temperature. Cells were ...read more
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and human ICAM-1/CD54 Ramos human Burkitt's lymphoma cell line (KO). PVDF membrane was probed with 1:1000 µg/mL of Goat Anti-Human ...read more
Requirement of NF-kappa B activation for cytokine-mediated reductions in TEER.A: Concentration-dependent inhibition of induction of ICAM-1 expression by I kappa K-beta -inhibitor Bay11, confirming the effect of Bay11 on ...read more
Requirement of NF-kappa B activation for cytokine-mediated reductions in TEER.A: Concentration-dependent inhibition of induction of ICAM-1 expression by I kappa K-beta -inhibitor Bay11, confirming the effect of Bay11 on ...read more
Kinetics and dose response of distinct changes to HDMEC barriers induced by TNF and IL-beta .A: Relationship of the early TNF-induced TEER increase to basal TEER levels. A plot of the percent increase over basal TEER ...read more
Effects of TNF on the actin cytoskeleton and MLC phosphorylation.A) Analysis of the effects of TNF on actin/CL5 co-localization. Post-confluent HDMEC monolayers immunostained with anti-CL5 and phalloidin-stained for ...read more
Requirement of NF-kappa B activation for cytokine-mediated reductions in TEER.A: Concentration-dependent inhibition of induction of ICAM-1 expression by I kappa K-beta -inhibitor Bay11, confirming the effect of Bay11 on ...read more
Effects of ROCK inhibitors H-1152 and Y-27632 on TNF-induced MLC phosphorylation, actin and CL5 reorganization and the fall in TEER.A) Immunoblot analysis of the effects of H-1152 (top) or Y-27632 (bottom) concentration ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ICAM-1/CD54 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑1/CD54
Extracellular domain
Specificity
Detects human ICAM‑1/CD54 in direct ELISAs and Western blots. ICAM‑1/CD54 has been screened using CHO cells transfected with cDNAs for ICAM-1, VCAM-1, and E-Selectin. ICAM‑1/CD54 was shown to be only reactive with ICAM-1.
Source
N/A
Isotype
Serum
Clonality
Polyclonal
Host
Goat
Gene
ICAM1
Purity Statement
N/A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1:600 dilution
  • Immunohistochemistry 1:300 dilution
  • Knockout Validated 1:1000 ug/mL
  • Western Blot 1:1000 dilution
Publications
Read Publications using
BBA17 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Serum.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute in 0.5 mL of sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (BBA17)(18)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(2)
IHC
(2)
IHC-P
(2)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(9)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using BBA17 Applications Species
Lindsey I. Sinclair, Asher Mohr, Mizuki Morisaki, Martin Edmondson, Selina Chan, A. Bone-Connaughton et al. Is later-life depression a risk factor for Alzheimer's disease or a prodromal symptom: a study using post-mortem human brain tissue? Alzheimers Res Ther 2023-09-12 [PMID: 37700368]
Justin E. Johnson, Declan McGuone, Mina L. Xu, Dan Jane-Wit, Richard N. Mitchell, Peter Libby et al. Coronavirus Disease 2019 (COVID-19) Coronary Vascular Thrombosis: Correlation with Neutrophil but Not Endothelial Activation The American Journal of Pathology 2023-01-01 [PMID: 34599881]
SC Teixeira, RJ Silva, JB Lopes-Mari, AO Gomes, MB Angeloni, ML Fermino, MC Roque-Barr, NM Silva, DAO Silva, JR Mineo, EAV Ferro, BF Barbosa Transforming growth factor (TGF)-beta1 and interferon (IFN)-gamma differentially regulate ICAM-1 expression and adhesion of Toxoplasma gondii to human trophoblast (BeWo) and uterine cervical (HeLa) cells Acta tropica, 2021-08-25;0(0):106111. 2021-08-25 [PMID: 34450063] (ICC, Western Blot, Human) ICC, Western Blot Human
Dongsic Choi, Gyeongyun Go, Dae‐Kyum Kim, Jaewook Lee, Seon‐Min Park, Dolores Di Vizio et al. Quantitative proteomic analysis of trypsin‐treated extracellular vesicles to identify the real‐vesicular proteins Journal of Extracellular Vesicles 2020-09-01 [PMID: 32489530]
Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells International Journal of Oncology 2019-04-09 [PMID: 31081048]
N Schultz, K Brännström, E Byman, S Moussaud, HM Nielsen, A Olofsson, M Wennström Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population ex�vivo and in�vitro Aging Cell, 2018-02-17;0(0):. 2018-02-17 [PMID: 29453790] (IHC, Human) IHC Human
Amogh Sivarapatna, Mahboobe Ghaedi, Yang Xiao, Edward Han, Binod Aryal, Jing Zhou et al. Engineered Microvasculature in PDMS Networks Using Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Cell Transplantation 2017-09-14 [PMID: 28901188]
Douglas G Walker, Lih-Fen Lue, Tiffany M Tang, Charles H Adler, John N Caviness, Marwan N Sabbagh, Geidy E Serrano, Lucia I Sue, Thomas G Beach Changes in CD200 and intercellular adhesion molecule-1 (ICAM-1) levels in brains of Lewy body disorder cases are associated with amounts of Alzheimer's pathology not ?-synuclein pathology. Neurobiology of Aging, 2017-03-16;0(0):1558-1497. 2017-03-16 [PMID: 28390825] (Western Blot, Human) Western Blot Human
Scott M. Sugden, Tram N. Q. Pham, Éric A. Cohen HIV-1 Vpu Downmodulates ICAM-1 Expression, Resulting in Decreased Killing of Infected CD4 + T Cells by NK Cells Journal of Virology 2017-04-15 [PMID: 28148794]
Kathleen R Chirco, S Scott Whitmore, Kai Wang, Lawrence A Potempa, Jennifer A Halder, Edwin M Stone et al. Monomeric C-reactive protein and inflammation in age-related macular degeneration The Journal of Pathology 2016-10-01 [PMID: 27376713]
Show All 18 Publications.

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Bioinformatics

Gene Symbol ICAM1