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ICAM-1/CD54 Antibody (MEM-111) [PE]

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Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [PE] [NB500-318PE] - A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
MEM-111
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

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ICAM-1/CD54 Antibody (MEM-111) [PE] Summary

Additional Information
Clone MEM-111 was used by HLDA to establish CD designation.
Immunogen
Raji human Burkitt's lymphoma cell line (NM_000201.2).
Specificity
The antibody MEM-111 reacts with an extracellular epitope CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (MEM-111) [PE]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (NB500-318PE) (0)

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Product General Protocols

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WB Video Protocol

FAQs for ICAM-1/CD54 Antibody (NB500-318PE). (Showing 1 - 1 of 1 FAQ).

  1. Do you have information or data for equine validation?
    • NB500-318PE has not yet been validated in equine. Unfortunately, since we have not yet tested this in equine, we do not have any information available. This product is a licensed clone, and it does appear that much of the validation on the species/applications came via publications.
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Bioinformatics

Gene Symbol ICAM1