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ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]

Images

 
ICAM‑1/CD54 was detected in live unfixed C32 human melanoma cell line using Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 5 µg/mL for 1 hour at 2° - 8° C. Cells were ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG ...read more
Western blot shows lysates of C32. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, B/N, ICC/IF
Clone
BBIG-I1 (11C81)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated] Summary

Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-25 ug/mL
  • Immunoprecipitation
  • Western Blot 2 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.8
using
BBA3 in the following applications:

Publications
Read Publications using
BBA3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Publications for ICAM-1/CD54 Antibody (BBA3)(81)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 17 applications: Bead-based Bioassay, Bioassay, Cell-based ELISA, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, Immunocytochemistry, Immunoprecipitation, Inhibition, Neutralization, Western Blot.


Filter By Application
Bead-based Bioassay
(1)
Bioassay
(2)
Cell-based ELISA
(1)
ELISA Capture
(1)
ELISA Development
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(8)
Functional Assay
(2)
ICC
(14)
IF
(1)
IHC
(4)
IHC-Fr
(1)
Immunocytochemistry
(2)
Immunoprecipitation
(2)
Inhibition
(1)
Neutralization
(16)
Western Blot
(8)
All Applications
Filter By Species
Human
(53)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 81. Show All 81 Publications.
Publications using BBA3 Applications Species
Liang, NW;Wilson, C;Davis, B;Wolf, I;Qyli, T;Moy, J;Beebe, DJ;Schnapp, LM;Kerr, SC;Faust, HE; Modeling lung endothelial dysfunction in sepsis-associated ARDS using a microphysiological system Physiological reports 2024-07-01 [PMID: 38981846] (Immunocytochemistry, Human) Immunocytochemistry Human
Chien, Y;Huang, XY;Yarmishyn, AA;Chien, CS;Liu, YH;Hsiao, YJ;Lin, YY;Lai, WY;Huang, SC;Lee, MS;Chiou, SH;Yang, YP;Chiou, GY; Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells Virus research 2024-07-01 [PMID: 38754785] (Neutralization, Human) Neutralization Human
Cacho-Navas, C;López-Pujante, C;Reglero-Real, N;Colás-Algora, N;Cuervo, A;Conesa, JJ;Barroso, S;de Rivas, G;Ciordia, S;Paradela, A;D'Agostino, G;Manzo, C;Feito, J;Andrés, G;Molina-Jiménez, F;Majano, P;Correas, I;Carazo, JM;Nourshargh, S;Huch, M;Millán, J; ICAM-1 nanoclusters regulate hepatic epithelial cell polarity by leukocyte adhesion-independent control of apical actomyosin eLife 2024-04-10 [PMID: 38597186] (Immunoprecipitation, Immunocytochemistry, Human) Immunoprecipitation, Immunocytochemistry Human
Kintos, DP;Salagiannis, K;Sgouros, A;Nikolaropoulos, SS;Topouzis, S;Fousteris, MA; Identification of new multi-substituted 1H-pyrazolo[3,4-c]pyridin-7(6H)-ones as soluble guanylyl cyclase (sGC) stimulators with vasoprotective and anti-inflammatory activities Bioorganic chemistry 2024-02-02 [PMID: 38335755] (ELISA Capture, Rat) ELISA Capture Rat
Howard, C;Joof, F;Hu, R;Smith, JD;Zheng, Y; Probing cerebral malaria inflammation in 3D human brain microvessels Cell reports 2023-10-10 [PMID: 37819760] (ICC, Human) ICC Human
Marlene Geyer, Lisa-Marie Gaul, Sabrina Luigia D`Agosto, Vincenzo Corbo, Karla Queiroz The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model Frontiers in Immunology 2023-05-02 [PMID: 37205118]
Haley Ehlers, Arnaud Nicolas, Frederik Schavemaker, Jeroen P. M. Heijmans, Martin Bulst, Sebastiaan J. Trietsch et al. Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration Frontiers in Immunology 2023-01-24 [PMID: 36761747]
Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 2023-12-31 [PMID: 36495563]
C Akoto, A Willis, CF Banas, JA Bell, D Bryant, C Blume, DE Davies, EJ Swindle IL-33 Induces an Antiviral Signature in Mast Cells but Enhances Their Permissiveness for Human Rhinovirus Infection Viruses, 2022-11-01;14(11):. 2022-11-01 [PMID: 36366528] (Neutralization, Human) Neutralization Human
Victor Delprat, Camille Huart, Olivier Feron, Fabrice Soncin, Carine Michiels The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis Frontiers in Oncology 2022-09-28 [PMID: 36248978]
Show All 81 Publications.

Reviews for ICAM-1/CD54 Antibody (BBA3) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using BBA3:
Filter by Applications
IF
(3)
ICC
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Human 01/20/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 21277303
SpeciesHuman
  5
reviewed by:
Verified Customer
ICC Human 01/20/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID: 23104645
SpeciesHuman
  5
reviewed by:
Verified Customer
IF Human 01/20/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 21164106
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Human 01/20/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 22560286
SpeciesHuman

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FAQs for ICAM-1/CD54 Antibody (BBA3) (0)

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Verified Customer
01/20/2015
Application: IF
Species: Human

 
Verified Customer
01/20/2015
Application: ICC
Species: Human

 
Verified Customer
01/20/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol ICAM1