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HYPB Antibody

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Flow Cytometry: HYPB Antibody [NB100-77334] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Flow Cytometry: HYPB Antibody [NB100-77334] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, CaSpecies Glossary
Applications ELISA, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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HYPB Antibody Summary

Immunogen
Peptide with sequence C-ERDPDKQTQNKE corresponding to internal region according to NP_054878.5.
Localization
Nuclear
Predicted Species
Mouse (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SETD2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
Publications
Read Publication using NB100-77334.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HYPB Antibody

  • FLJ22472
  • FLJ23184
  • FLJ45883
  • FLJ46217
  • HIF1
  • HIF-1EC 2.1.1.43
  • HIP-1
  • histone-lysine N-methyltransferase SETD2
  • hSET2
  • huntingtin interacting protein 1
  • Huntingtin yeast partner B
  • Huntingtin-interacting protein 1
  • Huntingtin-interacting protein B
  • HYPBp231HBP
  • KIAA1732SET domain-containing protein 2
  • KMT3AHBP231
  • Lysine N-methyltransferase 3A
  • SET domain containing 2
  • SET2FLJ16420

Background

Histone methylation plays an important role in eukaryotic transcriptional regulation. KMT3A / HYPB (huntingtin interacting protein B) also identified as HIF-1 (huntingtin interacting protein 1) is implicated in the pathogenesis of Huntington disease. KMT3A / HYPB coordinates histone methylation and transcriptional regulation in mammals. The HYPB domains, AWA-SET-PostSET, mediate histone H3 lysine 36 specific HMTase activity. The C-terminal of HYPB protein is important for its interaction with hyperphosphorylated RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-77334
HYPB Antibody
Species: Hu, Mu, Po, Ca
Applications: ELISA, Flow

Publications for HYPB Antibody (NB100-77334)(1)


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Bioinformatics

Gene Symbol SETD2
Entrez
Uniprot