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Human VEGF DuoSet ELISA, 15 Plate

 (196 Publications)
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Effects of cell stress on VEGF-A and HIF1 alpha expression in Müller cells and Y79 photoreceptors. (A) VEGF-A was measured by ELISA using conditioned media collected from MIO-M1 and Y79 cells, n = 4–5/group in ...read more

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human VEGF DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
VEGFA

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Reviewed Applications
Read 1 Review rated 4
using
DY293B in the following application:
Publications
Read Publications using
DY293B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VEGF DuoSet ELISA, 15 Plate

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3). 
VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size, but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). After birth, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (26).

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Publications for VEGF (DY293B)(196)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Ovine, Porcine, Xenograft.

We have publications tested in 2 applications: ChIP, ELISA.


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(1)
ELISA
(1)
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Human
(187)
Mouse
(2)
Rat
(3)
Bovine
(1)
Chicken
(1)
Ovine
(1)
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(2)
Xenograft
(1)
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Showing Publications 1 - 10 of 196. Show All 196 Publications.
Publications using DY293B Applications Species
Snuggs, JW;Senter, RK;Whitt, JP;Jackson, JD;Le Maitre, CL; PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration Gene therapy 2024-11-21 [PMID: 39572769] (Human) Human
Miyatani, T;Tanaka, H;Numa, K;Uehara, A;Otsuki, Y;Hamuro, J;Kinoshita, S;Sotozono, C; Clustered ARPE-19 cells distinct in mitochondrial membrane potential may play a pivotal role in cell differentiation Scientific reports 2024-09-27 [PMID: 39333742] (Human) Human
Delila, L;Nebie, O;Le, NTN;Timmerman, K;Lee, DY;Wu, YW;Chou, ML;Buée, L;Chou, SY;Blum, D;Devos, D;Burnouf, T; Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson's disease models Journal of biomedical science 2024-09-05 [PMID: 39237980] (Human) Human
McPhail, DK;Alzahrani, MAM;Martin, KR;Calver, BL;Harwood, AJ;MacKeigan, JP;Davies, DM;Tee, AR; Loss of Tuberous Sclerosis Complex 2 confers inflammation via dysregulation of Nuclear factor kappa-light-chain-enhancer of activated B cells Research square 2024-07-15 [PMID: 39070657] (Human) Human
Basu, B;Karwatka, M;China, B;McKibbin, M;Khan, K;Inglehearn, CF;Ladbury, JE;Johnson, CA; Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epithelium The Journal of biological chemistry 2024-07-13 [PMID: 39009342] (Human) Human
Zisman, D;Sabtan, H;Rahat, MM;Simanovich, E;Haddad, A;Gazitt, T;Feld, J;Slobodin, G;Kibari, A;Elias, M;Rahat, MA; Tofacitinib Regulates Endostatin via Effects on CD147 and Cathepsin S International journal of molecular sciences 2024-07-02 [PMID: 39000375] (Human) Human
Lin, YH;Chen, TM;Tsai, YL;Tsai, WC;Wang, HH;Chen, Y;Wu, ST; The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells International journal of molecular sciences 2024-05-20 [PMID: 38791597] (Human) Human
Jiang, J;Röper, L;Fuchs, F;Hanschen, M;Failer, S;Alageel, S;Cong, X;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Bone Regenerative Effect of Injectable Hypoxia Preconditioned Serum-Fibrin (HPS-F) in an Ex Vivo Bone Defect Model International journal of molecular sciences 2024-05-13 [PMID: 38791352] (Human) Human
Harary Søndergaard, R;Drozd Højgaard, L;Haack-Sørensen, M;Hoeeg, C;Mønsted Johansen, E;Follin, B;Kastrup, J;Ekblond, A;Juhl, M; Investigating the paracrine and juxtacrine abilities of adipose-derived stromal cells in angiogenesis triple cell co-cultures Stem cell research 2024-04-09 [PMID: 38608355] (Human) Human
López-Guzmán, C;García, AM;Marín, P;Vásquez, AM; Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture Methods and protocols 2024-02-15 [PMID: 38392690] (Human) Human
Show All 196 Publications.

Review for VEGF (DY293B) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using DY293B:
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  4
reviewed by:
Verified Customer
 06/28/2023
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Summary

Sample Testedhuman dermal fibroblast,Supernatant from cultured human dermal fibroblasts (HDFa)
LotP143535

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (DY293B). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.
Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.
mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.
Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.
Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.
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VEGF DY293B
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06/28/2023
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Bioinformatics

Gene Symbol VEGFA