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Human VEGF 165b DuoSet ELISA, 15 Plate

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human VEGF 165b DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
VEGFA

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY3045.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VEGF 165b DuoSet ELISA, 15 Plate

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

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Publications for VEGF (DY3045)(11)

We have publications tested in 2 confirmed species: Human, Ovine.


Filter By Application
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Filter By Species
Human
(10)
Ovine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using DY3045 Applications Species
Quan, B;Li, Z;Yang, H;Li, S;Yan, X;Wang, Y; The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF165 and downregulating VEGF165b Heliyon 2023-08-01 [PMID: 37554848] (Human) Human
V Tekin, T Aweda, O Kozgus Gul, FZ Biber Muft, J Bartels, SE Lapi, P Unak A novel anti-angiogenic radio/photo sensitizer for prostate cancer imaging and therapy: 89Zr-Pt@TiO2-SPHINX, synthesis and in vitro evaluation Nuclear medicine and biology, 2021-01-09;94(0):20-31. 2021-01-09 [PMID: 33482596] (Human) Human
D Mao, Y Hu, Q Bao, K Wu, Y Zheng, Y Yang, B Lei, Y Jiang Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients BMC Ophthalmol, 2020-08-05;20(1):319. 2020-08-05 [PMID: 32758187] (Human) Human
MM Karsten, MH Beck, A Rademacher, J Knabl, JU Blohmer, J Jückstock, JC Radosa, P Jank, B Rack, W Janni VEGF-A165b levels are reduced in breast cancer patients at primary diagnosis but increase after completion of cancer treatment Sci Rep, 2020-02-27;10(1):3635. 2020-02-27 [PMID: 32108136] (Human) Human
A Boudria, C Abou Fayca, T Jia, S Gout, M Keramidas, C Didier, N Lemaître, S Manet, JL Coll, AC Toffart, D Moro-Sibil, C Albiges-Ri, V Josserand, E Faurobert, C Brambilla, E Brambilla, S Gazzeri, B Eymin VEGF165b, a splice variant of VEGF-A, promotes lung tumor progression and escape from anti-angiogenic therapies through a ?1 integrin/VEGFR autocrine loop Oncogene, 2018-09-07;0(0):. 2018-09-07 [PMID: 30194450] (Human) Human
J Castle-Mil, DO Bates, DJ Tortonese Mechanisms regulating angiogenesis underlie seasonal control of pituitary function Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2514-E2523. 2017-03-07 [PMID: 28270617] (Ovine) Ovine
Bills V, Hamdollah-Zadeh M, Soothill P, Harper S, Bates D The role of VEGF-A165b in trophoblast survival. BMC Pregnancy Childbirth, 2014-08-15;14(0):278. 2014-08-15 [PMID: 25128406] (Human) Human
Chan , Kim H, Simpson , Philippa, Yong , Andy S, Dunn , Louise L, Chawantanpipat , Chirapan, Hsu , Chijen, Yu , Young, Keech , Anthony, Celermajer , David S, Ng , Martin K The relationship between endothelial progenitor cell populations and epicardial and microvascular coronary disease-a cellular, angiographic and physiologic study. PLoS ONE, 2014-04-15;9(4):e93980. 2014-04-15 [PMID: 24736282] (Human) Human
Clifford RL, John AE, Brightling CE Abnormal histone methylation is responsible for increased vascular endothelial growth factor 165a secretion from airway smooth muscle cells in asthma. J. Immunol., 2012-06-11;189(2):819-31. 2012-06-11 [PMID: 22689881] (Human) Human
Yamashita-Kashima Y, Fujimoto-Ouchi K, Yorozu K, Kurasawa M, Yanagisawa M, Yasuno H, Mori K Biomarkers for antitumor activity of bevacizumab in gastric cancer models. BMC Cancer, 2012-01-25;12(0):37. 2012-01-25 [PMID: 22273502] (Human) Human
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (DY3045). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Additional VEGF Products

Blogs on VEGF. Showing 1-10 of 23 blog posts - Show all blog posts.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol VEGFA