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Human EGF Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human EGF Quantikine ELISA Kit Summary

Background
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showe...d linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
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Specificity
Natural and recombinant human EGF
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
EGF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DEG00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human EGF Quantikine ELISA Kit

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for EGF (DEG00)(76)

We have publications tested in 3 confirmed species: Human, Rat, Plant - Arabidopsis.


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Human
(74)
Rat
(1)
Plant - Arabidopsis
(1)
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Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using DEG00 Applications Species
Lin, YK;Tanner, E;Wang, Y;Ye, W;Ang, L;Ju, W;Pop-Busui, R; Urinary epidermal growth factor levels correlate with cardiovascular autonomic neuropathy indices in adults with type 1 diabetes Journal of diabetes investigation 2023-07-03 [PMID: 37395013] (Human) Human
Yang, YC;Chien, Y;Yarmishyn, AA;Lim, LY;Tsai, HY;Kuo, WC;Tsai, PH;Yang, SH;Hong, SI;Chen, SJ;Hwang, DK;Yang, YP;Chiou, SH; Inhibition of oxidative stress-induced epithelial-mesenchymal transition in retinal pigment epithelial cells of age-related macular degeneration model by suppressing ERK activation Journal of advanced research 2023-06-14 [PMID: 37328058] (Human) Human
Oh, SJ;Lim, JY;Son, MK;Ahn, JH;Song, KH;Lee, HJ;Kim, S;Cho, EH;Chung, JY;Cho, H;Kim, H;Kim, JH;Park, J;Choi, J;Hwang, SW;Kim, TW; TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway Nature communications 2023-05-10 [PMID: 37165076] (Human) Human
Katabami, M;Kinoshita, I;Ariga, S;Shimizu, Y;Dosaka-Akita, H; Crystalline silica-exposed human lung epithelial cells presented enhanced anchorage-independent growth with upregulated expression of BRD4 and EZH2 in autocrine and paracrine manners PloS one 2023-05-05 [PMID: 37146018] (Human) Human
V Sabrina, C Carmen, A Gloria, B Elisa, C Nico, B Marina, F Luigi, V Piera Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing International Journal of Molecular Sciences, 2022-09-14;23(18):. 2022-09-14 [PMID: 36142617] (Human) Human
P Ngamjanyap, S Worawichaw, P Pisitkun, K Khiewngam, S Kantachuve, A Nongnuch, M Assanatham, N Sathirapon, C Kitiyakara Predicting treatment response and clinicopathological findings in lupus nephritis with urine epidermal growth factor, monocyte chemoattractant protein-1 or their ratios PLoS ONE, 2022-03-10;17(3):e0263778. 2022-03-10 [PMID: 35271583] (Human) Human
E Razani, HR Aghayan, S Alavi-Mogh, F Yari, A Gharehbagh, Z Sharifi A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture Journal of virological methods, 2022-01-29;0(0):114478. 2022-01-29 [PMID: 35101406] (Human) Human
DH Shin, SH Kim, M Choi, YK Bae, C Han, BK Choi, SS Kim, JY Han Oncogenic KRAS promotes growth of lung cancer cells expressing SLC3A2-NRG1 fusion via ADAM17-mediated shedding of NRG1 Oncogene, 2021-11-06;0(0):. 2021-11-06 [PMID: 34743207] (Human) Human
L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 2021-09-23;0(0):. 2021-09-23 [PMID: 34556797] (Human) Human
HP Li, CY Huang, KW Lui, YK Chao, CN Yeh, LY Lee, Y Huang, TL Lin, YC Kuo, MY Huang, YR Lai, YM Yeh, HC Fan, AC Lin, JC Hsieh, KP Chang, CY Lin, HM Wang, YS Chang, CL Hsu Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment Cancers, 2021-06-12;13(12):. 2021-06-12 [PMID: 34204797] (Human) Human
Show All 76 Publications.

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Product General Protocols

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Additional EGF Products

Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

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Bioinformatics

Gene Symbol EGF