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Human CCL5/RANTES DuoSet ELISA, 15 Plate

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human CCL5/RANTES DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CCL5

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY278.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL5/RANTES DuoSet ELISA, 15 Plate

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), also known as CCL5, is a member of the "CC" subfamily of chemokines. It plays a primary role in the inflammatory immune response via its ability to chemoattract leukocytes and modulate their function. The cDNA for RANTES was initially discovered by subtractive hybridization as a T cell specific sequence (1, 2). Human RANTES cDNA encodes a highly basic 91 amino acid (aa) residue precursor polypeptide with a 23 aa hydrophobic signal peptide that is cleaved to generate the 68 aa mature protein (1, 2). Human RANTES exhibits approximately 85% homology with mouse RANTES at the deduced aa level (3, 4). 
RANTES is a potent chemoattractant for a number of different cell types including unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with naive and memory phenotypes, NK cells, basophils, eosinophils, dendritic cells, mast cells, monocytes, and microglia (5-13). In addition to its effects on migration, RANTES can activate a number of cell types including T cells (14-16), monocytes (17), neutrophils (17), NK cells (7), dendritic cells (18), and astrocytes (19). T cell activation generally requires relatively high RANTES concentrations (~ 1 μM) and is dependent upon aggregation of the molecule and association with cell surface glycosaminoglycans (GAGs) (15-17). Whether this activity occurs in vivo remains unclear although in mice, intraperitoneally injected RANTES mutants that are unable to aggregate and/or bind GAG, are not capable of attracting leukocytes when compared to wild-type controls (20). Other in vivo studies show that RANTES knockout mice exhibit deficient recruitment of leukocytes to sites of acute inflammation (21). 
RANTES, is known to interact with four identified seven transmembrane G-protein coupled receptors: CCR1, CCR3, CCR4, and CCR5 (22-25). RANTES stimulation can initiate a variety of signaling cascades that are cell context dependent. For instance, in T-cells, RANTES can stimulate elevations of intracellular Ca2+ (26), and activation of focal adhesion kinase (FAK) (27), protein kinase A (28), PI3-kinase (14), Rho GTPase (29), and JAK/STAT signaling pathways (30). The cytomegalovirus protein US28 exhibits significant homology with CC chemokine receptors and is capable of binding RANTES (31). Membrane-spanning US28 can, depending on the context, signal in a constitutive manner (32), bind RANTES and initiate G-protein-mediated signaling cascades (33), or sequester RANTES and potentially alter inflammatory responses (34-36). 
The RANTES receptor CCR5 is also the primary co-receptor for R5 (M-tropic) variants of HIV-1 (37, 38). It has been demonstrated that RANTES, as well as the other CCR5 ligands, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta , can competitively inhibit CCR5/HIV-1 interaction and suppress viral infection in vitro (39, 40). These effects apparently do not require fully intact signaling from the CCR5 receptor (41). Consequently, modified forms of RANTES and non-peptide compounds that block the interaction of HIV-1 with CCR5 show promise for future therapies (41-44). In contrast, several reports show that RANTES can enhance in vitro replication of X4 (T-tropic) variants of HIV-1 that use CXCR4 as a co-receptor rather than CCR5 (45, 46). This activity usually requires relatively high RANTES concentrations (~μM) and is dependent upon interaction with cell surface GAGs, oligomerization, and activation of tyrosine kinase and MAP kinase signaling cascades (46, 47).

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Publications for CCL5/RANTES (DY278)(66)

We have publications tested in 1 confirmed species: Human.


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(66)
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Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using DY278 Applications Species
Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human) Human
Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P; Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders Psychoneuroendocrinology 2024-07-06 [PMID: 39003840] (Human) Human
Boeszoermenyi, A;Bernaleau, L;Chen, X;Kartnig, F;Xie, M;Zhang, H;Zhang, S;Delacrétaz, M;Koren, A;Hopp, AK;Dvorak, V;Kubicek, S;Aletaha, D;Yang, M;Rebsamen, M;Heinz, LX;Superti-Furga, G; A conformation-locking inhibitor of SLC15A4 with TASL proteostatic anti-inflammatory activity Nature communications 2023-10-20 [PMID: 37863876] (Human) Human
Hjell, G;Rokicki, J;Szabo, A;Holst, R;Tesli, N;Bell, C;Fischer-Vieler, T;Werner, MCF;Lunding, SH;Ormerod, MBEG;Johansen, IT;Djurovic, S;Ueland, T;Andreassen, OA;Melle, I;Lagerberg, TV;Mørch-Johnsen, L;Steen, NE;Haukvik, UK; Impulsivity across severe mental disorders: a cross-sectional study of immune markers and psychopharmacotherapy BMC psychiatry 2023-09-07 [PMID: 37674162] (Human) Human
Watzling, M;Klaus, L;Weidemeier, T;Horder, H;Ebert, R;Blunk, T;Bauer-Kreisel, P; Three-Dimensional Breast Cancer Model to Investigate CCL5/CCR1 Expression Mediated by Direct Contact between Breast Cancer Cells and Adipose-Derived Stromal Cells or Adipocytes Cancers 2023-07-05 [PMID: 37444610] (Human) Human
Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C; Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease International journal of molecular sciences 2023-06-20 [PMID: 37373554] (Human) Human
Kislat, A;Olah, P;Kuchner, M;Gerber, PA;Schrader, J;Meller, S;Homey, B; The Endogenous Dual Retinoid Receptor Agonist Alitretinoin Exhibits Immunoregulatory Functions on Antigen-Presenting Cells International journal of molecular sciences 2023-06-02 [PMID: 37298605] (Human) Human
Aguilera-Herce, J;Panadero-Medianero, C;S�nchez-Romero, MA;Balbont�n, R;Bernal-Bayard, J;Ramos-Morales, F; Salmonella Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells International journal of molecular sciences 2023-05-07 [PMID: 37176110] (Human) Human
D Zimmerli, CS Brambillas, F Talens, J Bhin, R Linstra, L Romanens, A Bhattachar, SEP Joosten, AM Da Silva, N Padrao, MD Wellenstei, K Kersten, M de Boo, M Roorda, L Henneman, R de Bruijn, S Annunziato, E van der Bu, AP Drenth, C Lutz, T Endres, M van de Ven, M Eilers, L Wessels, KE de Visser, W Zwart, RSN Fehrmann, MATM van Vugt, J Jonkers MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling Nature Communications, 2022-11-02;13(1):6579. 2022-11-02 [PMID: 36323660] (Human) Human
S Guerrero, E Sánchez-Ti, L Agüí, A González-C, P Yáñez-Sede, JM Pingarrón Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis Biosensors, 2022-08-07;12(8):. 2022-08-07 [PMID: 36005006] (Human) Human
Show All 66 Publications.

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Bioinformatics

Gene Symbol CCL5