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HSP90 beta Antibody (HSP90AB1/4970)

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Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta antibody (HSP90AB1/4970). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta antibody (HSP90AB1/4970). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human thyroid stained with HSP90 beta antibody (HSP90AB1/4970). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
HSP90AB1/4970
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20694AF350
Alexa Fluor 405
NBP3-20694AF405
Alexa Fluor 488
NBP3-20694AF488
Alexa Fluor 532
NBP3-20694AF532
Alexa Fluor 594
NBP3-20694AF594
Alexa Fluor 647
NBP3-20694AF647
Alexa Fluor 700
NBP3-20694AF700
Alexa Fluor 750
NBP3-20694AF750
Allophycocyanin
NBP3-20694APC
Biotin
NBP3-20694B
CoraFluor 1
NBP3-20694CL1
DyLight 350
NBP3-20694UV
DyLight 405
NBP3-20694V
DyLight 488
NBP3-20694G
DyLight 550
NBP3-20694R
DyLight 594
NBP3-20694DL594
DyLight 650
NBP3-20694C
DyLight 680
NBP3-20694FR
DyLight 755
NBP3-20694IR
FITC
NBP3-20694F
HRP
NBP3-20694H
Janelia Fluor 525
NBP3-20694JF525
Janelia Fluor 549
NBP3-20694JF549
Janelia Fluor 585
NBP3-20694JF585
Janelia Fluor 635
NBP3-20694JF635
Janelia Fluor 646
NBP3-20694JF646
Janelia Fluor 669
NBP3-20694JF669
PE
NBP3-20694PE
PerCP
NBP3-20694PCP
Unconjugated
NBP3-20694
mFluor Violet 450 SE
NBP3-20694MFV450
mFluor Violet 500 SE
NBP3-20694MFV500
mFluor Violet 610 SE
NBP3-20694MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20694
BSA Free
NBP3-20694
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HSP90 beta Antibody (HSP90AB1/4970) Summary

Description
Positive Controls: Human spleen, stomach or pancreas tissue. MCF-7 cells.

Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Immunogen
Recombinant full-length human HSP90 beta protein
Localization
Cytoplasm.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for HSP90 beta Antibody (HSP90AB1/4970)

  • D6S182
  • Heat shock 84 kDa
  • heat shock 90kD protein 1, beta
  • heat shock 90kDa protein 1, beta
  • heat shock protein 90kDa alpha (cytosolic), class B member 1
  • heat shock protein beta
  • heat shock protein HSP 90-beta
  • HSP 84
  • HSP84
  • HSP90 beta
  • HSP90AB1
  • HSP90B
  • HSP90-BETA
  • HSP90BHSP 90
  • HSPC2
  • HSPC2D6S182
  • HSPCB
  • HSPCBFLJ26984

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HSP90 beta
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NBP3-20590
HSP90 beta Antibody (HSP90AB1/4970)
Species: Hu, Mu, Rt, Ca, Ch, Rb
Applications: WB, Flow, ICC/IF, IHC

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSP90AB1