HSP90 beta Antibodies
We offer HSP90 beta Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, SDS-Page, Simple Western, Western Blot. Each HSP90 beta Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSP90 beta Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Primate, Rabbit, Rat. Use the list below to choose the HSP90 beta Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSP90 beta polyclonal antibodies and browse our HSP90 beta monoclonal antibody catalog.
Alternate Names for HSP90 beta Antibodies
anti-HSP90 beta antibody, anti-HSP90AB1 antibody, anti-Heat shock 84 kDa antibody, anti-heat shock 90kD protein 1, beta antibody, anti-heat shock 90kDa protein 1, beta antibody, anti-heat shock protein 90kDa alpha (cytosolic), class B member 1 antibody, anti-heat shock protein beta antibody, anti-heat shock protein HSP 90-beta antibody, anti-HSP 84 antibody, anti-HSP84 antibody, anti-HSP90-BETA antibody, anti-HSP90BHSP 90 antibody, anti-HSPC2D6S182 antibody, anti-HSPCBFLJ26984 antibody
21 Results for "hsp90-beta" in Primary Antibodies
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0040.jpg "Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunocytochemistry-Immunofluorescence-NB110-61640-img0034.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0044.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0031.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0039.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0041.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0042.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0043.jpg)
![Knockdown Validated: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP1-77563-img0008.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP1-77563] - Analysis of HSP901b in: 1. HeLa, 2. Ntera 2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. PC12 and 8. COS7.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Western-Blot-NBP1-77563-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP1-77563] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77563-img0006.jpg)
![Immunohistochemistry: HSP90 beta Antibody [NBP1-77563] - Staining of HSP901b in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Immunohistochemistry-NBP1-77563-img0004.jpg)
![Simple Western: HSP90 beta Antibody [NBP1-77563] - Simple Western lane view shows a specific band for Hsp90B in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Simple-Western-NBP1-77563-img0007.jpg)
![Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Western-Blot-NBP2-37590-img0002.jpg "Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunocytochemistry-Immunofluorescence-NBP2-37590-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of paraffin-embedded kidney cancer tissues (left) and brain tissues (right) using HSP90AB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunohistochemistry-NBP2-37590-img0005.jpg)
![Flow Cytometry: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Flow-Cytometry-NBP2-37590-img0004.jpg)
![ELISA: HSP90 beta Antibody (5G4) [NBP2-37590] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-ELISA-NBP2-37590-img0001.jpg)
![Western Blot: HSP90AB1 Antibody [NBP2-68978] - Analysis using Anti-HSP90AB1 antibody NBP2-68978 (A) shows similar pattern to independent antibody NBP2-55509 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-68978-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HSP90AB1 Antibody [NBP2-68978] - Analysis using Anti-HSP90AB1 antibody NBP2-68978 (A) shows similar pattern to independent antibody NBP2-55509 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68978] - Staining of human kidney.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68978-img0007.jpg)
![Immunohistochemistry: HSP90AB1 Antibody [NBP2-68978] - Immunohistochemical staining of human tonsil shows strong positivity in germinal center cells.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-NBP2-68978-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68978] - Staining of human adrenal gland, cervix, uterine, kidney and testis using Anti-HSP90AB1 antibody NBP2-68978 (A) shows similar protein distribution across tissues to independent antibody NBP2-68937 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68978-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68978] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68978-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68978] - Staining of human cervix, uterine.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68978-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68978] - Staining of human testis.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68978-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp90B Antibody [NBP1-77561] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp90B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77561-img0003.jpg "Immunocytochemistry/Immunofluorescence: Hsp90B Antibody [NBP1-77561] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: HSP90 beta Antibody [NBP1-77561] - Hsp90B Antibody [NBP1-77561] - Staining of HSP901b in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Immunohistochemistry-NBP1-77561-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland, cervix, uterine, kidney and testis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar protein distribution across tissues to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland, cervix, uterine, kidney and testis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar protein distribution across tissues to independent antibody NBP2-68978 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: HSP90 beta Antibody [NBP2-68937] - HSP90AB1 Antibody [NBP2-68937] - Analysis using Anti-HSP90AB1 antibody NBP2-68937 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-68937-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human cervix, uterine.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human kidney.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human testis.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP90AB1 Antibody [NBP2-68937] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunohistochemistry-Paraffin-NBP2-68937-img0008.jpg)
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-262202415355731.jpg "Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-26220241522415.jpg)
![Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate. All lanes: HSP90 beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 80-90 kDa](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202411485820.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-28220241312214.jpg)
![Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202414312789.jpg)
![Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-278202415212484.jpg "Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)
![Knockout Validated: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP2-55509-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Analysis using Anti-HSP90AB1 antibody NBP2-55509 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-55509-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55509-img0001.jpg)