| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant rat HSPH1 Val595-Asp733 Accession # Q66HA8 |
| Specificity | Detects human, mouse, and rat HSPH1. This antibody is predicted to react with HSPH1 alpha and beta isoforms based on sequence identity of the immunogen. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | HSPH1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta . HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. alpha and beta have calculated molecular weights of 96.4 kDa and 92 kDa, respectively, but an apparent molecular weight of 105‑110 kDa in SDS‑PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.
| Publication using AF4029 | Applications | Species |
|---|---|---|
| Hu, L;Sun, C;Kidd, JM;Han, J;Fang, X;Li, H;Liu, Q;May, AE;Li, Q;Zhou, L;Liu, Q; A first-in-class inhibitor of Hsp110 molecular chaperones of pathogenic fungi Nature communications 2023-05-12 [PMID: 37173314] (Western Blot, N/A) | Western Blot | N/A |
Secondary Antibodies |
Isotype Controls |
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