HSP90 beta Antibody (H9010)

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta; exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1'2% ofcytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: KD, WB.

Showing Publications 1 - 5 of 5.

Publications using NB110-61640	Applications	Species
Muyin Liu, Su Li, Ming Yin, Youran Li, Jinxiang Chen, Yuqiong Chen, You Zhou, Qiyu Li, Fei Xu, Chunfeng Dai, Yan Xia, Ao Chen, Danbo Lu, Zhangwei Chen, Juying Qian, Junbo Ge Pinacidil ameliorates cardiac microvascular ischemia–reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin Basic Research in Cardiology 2024-01-02 [PMID: 38168863]
YU D, Yao S, Xi W et al. HSC70?HSPA1A, and HSP90AB1 Facilitate Ebola Virus trVLPs to Induce Chaperone-Mediated Autophagy Research Square 2023-08-18
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
Yu D, Yao S, Xi W et al. HSC70, HSPA1A, and HSP90AB1 Facilitate Ebola Virus trVLPs to Induce Autophagy bioRxiv 2020-06-22 (WB, KD, Human)	WB, KD	Human
Guo B, Xu P, Chai D et al. gB co-immunization with GP96 enhances pulmonary-resident CD8 T cells and exerts a long-term defence against MCMV pneumonitis J Cell Mol Med 2020-11-06 [PMID: 33155438] (WB, Mouse) Details: Western blot analysis of 3T3 cells transfected with pGP96-NT plasmids.	WB	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	04/04/2020	View Summary Application Western Blot Sample Tested HepG2,Human liver cancer cell line (HuH7) Species Human Lot 0811
Enlarge	5 reviewed by: Verified Customer	IHC-P	Human	04/04/2020	View Summary Application Immunohistochemistry-Paraffin Sample Tested Breast cancer tissue Species Human Lot 0811
Enlarge	5 reviewed by: Verified Customer	IHC-P	Human	02/19/2019	View Summary Application Immunohistochemistry-Paraffin Sample Tested Breast cancer tissue Species Human Lot 0811
Enlarge	4 reviewed by: Verified Customer	WB	Human	12/19/2018	View Summary Application Western Blot Sample Tested 293T,hela cell Species Human Lot 0811
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/17/2018	View Summary Application Western Blot Sample Tested A549 cells,HCC827 human non-small cell lung cancer cell line Species Human Lot 0811

Array NB110-61640

• HSP90 beta Antibodies
• HSP90 beta ELISA Kits
• HSP90 beta Lysate
• HSP90 beta Proteins
• HSP90 beta ELISA