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Hsp47 Antibody (SR1796)

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Western Blot: Hsp47 Antibody (SR1796) [NBP3-21700] - Western blot analysis using NBP3-21700 in HeLa cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
SR1796
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Hsp47 Antibody (SR1796) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Hsp47 (Uniprot #: P50454)
Specificity
Detects endogenous levels of total Hsp47
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SERPINH1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Hsp47 Antibody (SR1796)

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SERPINH1