Hsp47 Antibodies
We offer Hsp47 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each Hsp47 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Hsp47 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep. Use the list below to choose the Hsp47 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Hsp47 polyclonal antibodies and browse our Hsp47 monoclonal antibody catalog.
Alternate Names for Hsp47 Antibodies
anti-Hsp47 antibody, anti-SERPINH1 antibody, anti-(collagen binding protein 1) antibody, anti-Arsenic-transactivated protein 3 antibody, anti-AsTP3 antibody, anti-CBP1 antibody, anti-CBP2RA-A47 antibody, anti-Cell proliferation-inducing gene 14 protein antibody, anti-Collagen-binding protein antibody, anti-colligen antibody, anti-Colligin antibody, anti-colligin-1 antibody, anti-colligin-2 antibody, anti-gp46 antibody, anti-HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47) antibody, anti-member 1, (collagen binding protein 1) antibody, anti-member 2 antibody, anti-member 2, (collagen-binding protein 2) antibody, anti-PPROM antibody, anti-proliferation-inducing gene 14 antibody, anti-rheumatoid arthritis antigen A-47,47 kDa heat shock protein antibody, anti-Rheumatoid arthritis-related antigen RA-A47 antibody, anti-serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47) antibody, anti-serpin H1 antibody, anti-serpin peptidase i
11 Results for "hsp47" in Primary Antibodies
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0006.jpg "Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT and BMP signaling pathways play crucial roles in regeneration. a-d Real-time PCR for WNT and BMP signals, including Wnt7b Dual immunostaining for K15 and phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar?=?50?um. h, i Immunostaining and confocal microscope. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09402-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunocytochemistry-Immunofluorescence-NBP1-97491-img0007.jpg)
![Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Frozen-NBP1-97491-img0004.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0001.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0002.jpg)
![Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Simple-Western-NBP1-97491-img0005.jpg)
![Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SERPINH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP1-88172-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SERPINH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Hsp47 Antibody [NBP1-88172] - Staining of human cell line U-2 OS shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88172-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP1-88172] - Staining of human vulva/anal skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP1-88172-img0009.jpg)
![Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP1-88172-img0008.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0008.jpg "Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.")
![Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Immunoperoxidase of monoclonal antibody to SERPINH1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 5 ug/ml]](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Immunohistochemistry-Paraffin-H00000871-M01-img0002.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2 Analysis of SERPINH1 expression in HeLa.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0005.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in MCF-7.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0006.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in human pancreas.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0007.jpg)
![Sandwich ELISA: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Detection limit for recombinant GST tagged SERPINH1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Sandwich-ELISA-H00000871-M01-img0004.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0007.jpg "Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded mouse kidney. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0003.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0001.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug A431 whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0006.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded human breast carcinoma. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0004.jpg)
![Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Western-Blot-NBP3-15430-img0003.jpg "Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunofluorescence analysis of NIH-3T3 cells using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Immunocytochemistry-Immunofluorescence-NBP3-15430-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunohistochemistry of paraffin-embedded human colon using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Immunohistochemistry-Paraffin-NBP3-15430-img0001.jpg)
![Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Western-Blot-NBP2-22400-img0001.jpg "Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0003.jpg)
![Western Blot: Hsp47 Antibody (SR1796) [NBP3-21700] - Western blot analysis using NBP3-21700 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21700_rabbit-hsp47-mab-sr1796-57202317161867.jpg "Western Blot: Hsp47 Antibody (SR1796) [NBP3-21700] - Western blot analysis using NBP3-21700 in HeLa cell lysate.")
![Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163] - IHC image of Hsp47 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Hsp47-Antibody-10B2-Immunohistochemistry-NBP3-15163-img0001.jpg "Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163] - IHC image of Hsp47 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")