![Western Blot: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HMGN3-TRIP7-Antibody-Western-Blot-NB100-59840-img0003.jpg "Western Blot: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 60 and 99 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HMGN3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry ERMAP Antibody [Unconjugated]](https://images.novusbio.com/images2/ERMAP_AF4928_Flow_Cytometry_11957.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for HMGN3/TRIP7 Antibody (NB100-59840)Find related products by research area.
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| Gene Symbol | HMGN3 |
| Entrez |
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| Uniprot |
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![Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59840). Detection: DAB](http://images.novusbio.com/fullsize/HMGN3-TRIP7-Antibody-Immunohistochemistry-NB100-59840-img0004.jpg "Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59840). Detection: DAB")
![Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59839 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HMGN3-TRIP7-Antibody-Immunoprecipitation-NB100-59840-img0005.jpg "Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59839 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Immunocytochemistry/ Immunofluorescence Cathepsin B Antibody [Unconjugated]](https://images.novusbio.com/images/af965_mouse-cathepsin-b-affinity-purified-polyclonal-ab-822024950321.jpg)
![Immunocytochemistry/ Immunofluorescence Cathepsin B Antibody [Unconjugated]](https://images.novusbio.com/images/af965_mouse-cathepsin-b-affinity-purified-polyclonal-ab-822024950468.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")