![Immunohistochemistry-Paraffin: HMGN3/TRIP7 Antibody [NB100-59839] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59839). Detection: DAB](http://images.novusbio.com/fullsize/HMGN3-TRIP7-Antibody-Immunohistochemistry-Paraffin-NB100-59839-img0004.jpg "Immunohistochemistry-Paraffin: HMGN3/TRIP7 Antibody [NB100-59839] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59839). Detection: DAB")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 30 and 60 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324). |
| Predicted Species | Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HMGN3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HMGN3/TRIP7 Antibody (NB100-59839)Find related products by research area.
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| Gene Symbol | HMGN3 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59839] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59839 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59840 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HMGN3-TRIP7-Antibody-Immunoprecipitation-NB100-59839-img0005.jpg "Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59839] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59839 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59840 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")