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HLA DQB1 Antibody - Azide and BSA Free

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Western Blot: HLA DQB1 Antibody [NBP3-16006] - Western blot analysis of extracts of Raji cells, using HLA DQB1 antibody (NBP3-16006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry-Paraffin: HLA DQB1 Antibody [NBP3-16006] - Immunohistochemistry of paraffin-embedded human colon carcinoma using HLA DQB1 antibody (NBP3-16006) at dilution of 1:100 (40x lens).Perform high pressure ...read more
Immunohistochemistry-Paraffin: HLA DQB1 Antibody [NBP3-16006] - Immunohistochemistry of paraffin-embedded human tonsil using HLA DQB1 antibody (NBP3-16006) at dilution of 1:100 (40x lens).Perform high pressure antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Catalog# & Formulation Size Price
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HLA DQB1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 33-127 of human HLA DQB1 (NP_002114.3). RDSPEDFVFQFKGMCYFTNGTERVRLVTRYIYNREEYARFDSDVGVYRAVTPQGRPDAEYWNSQKEVLEGTRAELDTVCRHNYEVAFRGILQRRV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLA-DQB1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for HLA DQB1 Antibody - Azide and BSA Free

  • DC-1 alpha chain
  • DC-alpha
  • HLA class II histocompatibility antigen, DQ alpha 1 chain-like
  • HLA-DCA
  • MHC class II DQA1

Background

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-DQB1