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GRASP65 Antibody

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Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Confocal immunofluorescence analysis of U2OS cells using GRASP65 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Confocal immunofluorescence analysis of U2OS cells using GRASP65 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: GRASP65 Antibody [NBP3-38297] - Western blot analysis of various lysates using GRASP65 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Immunofluorescence analysis of A549 cells using GRASP65 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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GRASP65 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GRASP65 (NP_114105.1).

Sequence:
ALPLGAPPPDALPPGPTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPSHSAPDPDGLPHFMETPLQPPPPVQRVMDPGFLDVSGISLLDNSNASVWPSLPSSTELTTTAVSTSGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GORASP1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:500 - 1:2000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GRASP65 Antibody

  • Golgi peripheral membrane protein p65
  • Golgi phosphoprotein 5MGC118897
  • golgi reassembly stacking protein 1, 65kDa
  • Golgi reassembly-stacking protein 1
  • Golgi reassembly-stacking protein of 65 kDa
  • GRASP65MGC118894
  • P6565 kDa

Background

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GORASP1