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GRAP2 Antibody (1G12)

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Western Blot: GRAP2 Antibody (1G12) [H00009402-M01] - GRAP2 monoclonal antibody (M01), clone 1G12 Analysis of GRAP2 expression in Jurkat.
Western Blot: GRAP2 Antibody (1G12) [H00009402-M01] - GRAP2 monoclonal antibody (M01), clone 1G12 Analysis of GRAP2 expression in Jurkat.
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of monoclonal antibody to GRAP2 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
Western Blot: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of GRAP2 expression in transfected 293T cell line by GRAP2 monoclonal antibody (M01), clone 1G12.Lane 1: GRAP2 transfected lysate(38 KDa).Lane 2: ...read more
Immunoprecipitation: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of GRAP2 transfected lysate using anti-GRAP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GRAP2 monoclonal antibody.
ELISA: GRAP2 Antibody (1G12) [H00009402-M01] - Detection limit for recombinant GST tagged GRAP2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
1G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GRAP2 Antibody (1G12) Summary

Immunogen
GRAP2 (AAH25692, 226 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HHFHQERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALYDFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHN
Specificity
GRAP2 - GRB2-related adaptor protein 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GRAP2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GRAP2 Antibody (1G12)

  • Adapter protein GRID
  • GADS
  • GADSSH3-SH2-SH3 adapter Mona
  • GRAP2
  • GRB2L
  • GRB-2-like protein
  • GRB2-related adapter protein 2
  • GRB2-related adaptor protein 2
  • GRB2-related protein with insert domain
  • GRBLG
  • GRBLGGRAP-2
  • GRBX
  • Grf40 adapter protein
  • Grf40
  • grf-40
  • GRID
  • GRIDGRPL
  • Growth factor receptor-binding protein
  • growth factor receptor-bound protein 2-related adaptor protein 2
  • GRPL
  • Hematopoietic cell-associated adapter protein GrpL
  • hematopoietic cell-associated adaptor protein GRPL
  • Mona
  • P38
  • Protein GADS
  • SH3-SH2-SH3 adaptor molecule

Background

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GRAP2
Entrez
OMIM
Uniprot