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GR/NR3C1 Antibody (GT1073)

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Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. ...read more
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Rat brain. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Mouse intestine. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC
Clone
GT1073
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GR/NR3C1 Antibody (GT1073) Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human GR/NR3C1. The exact sequence is proprietary.
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR3C1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (88%), Cat (89%), Pig (83%), Sheep (89%), Guinea pig (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for GR/NR3C1 Antibody (GT1073)

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR3C1