![Western Blot: GLUT10 Antibody [NB100-490] - NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using NB100-490.](http://images.novusbio.com/fullsize/GLUT10-Antibody-Western-Blot-NB100-490-img0014.jpg "Western Blot: GLUT10 Antibody [NB100-490] - NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using NB100-490.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide containing residues 367-385 (ILSTAKKTKPHPRSGDPSA) of the human GLUT10 protein. |
| Specificity | Human GLUT10 protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC2A10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody has been tested in Western blot and immunofluorescence. No other applications have been tested. Western blot - Use at 1:500 to 1:1,000 dilution. *The investigator should determine the optimal dilution. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GLUT10 Antibody (NB100-490)Find related products by research area.
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| Gene Symbol | SLC2A10 |
![Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.](http://images.novusbio.com/fullsize/GLUT10-Antibody-Immunocytochemistry-Immunofluorescence-NB100-490-img0008.jpg "Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.")
![Western Blot: GLUT10 Antibody [NB100-490] - Cochlea from embryonic mice and mice up to 1 yr old were removed and the pure membrane fraction loaded on 4-20% SDS gel. Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4C with NB100-490 at 1:500 dilution followed by incubation with secondary Ab at 1:3,000 dilution for 1h at room temp. Protein bands were visualized on x-ray film with SuperSignal west femto ECL substrate. Lane 1:10ug of protein from liver Lane 2:10ug of protein from cochlea (E18) Lane 3:10ug of protein from cochlea (P7) Lane 4:10ug of protein from cochlea (P10) Lane 5:10ug of protein from cochlea (P14) Lane 6:10ug of protein from cochlea (P17) Lane 7:10ug of protein from cochlea (P60) Lane 8:10ug of protein from cochlea (1 Year)](http://images.novusbio.com/fullsize/GLUT10-Antibody-Western-Blot-NB100-490-img0012.jpg "Western Blot: GLUT10 Antibody [NB100-490] - Cochlea from embryonic mice and mice up to 1 yr old were removed and the pure membrane fraction loaded on 4-20% SDS gel. Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4C with NB100-490 at 1:500 dilution followed by incubation with secondary Ab at 1:3,000 dilution for 1h at room temp. Protein bands were visualized on x-ray film with SuperSignal west femto ECL substrate. Lane 1:10ug of protein from liver Lane 2:10ug of protein from cochlea (E18) Lane 3:10ug of protein from cochlea (P7) Lane 4:10ug of protein from cochlea (P10) Lane 5:10ug of protein from cochlea (P14) Lane 6:10ug of protein from cochlea (P17) Lane 7:10ug of protein from cochlea (P60) Lane 8:10ug of protein from cochlea (1 Year)")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")