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Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

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ELISA: Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) [NBP2-82143] - Samples were spiked with high concentrations of Rat Ghrelin/Obestatin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) [NBP2-82143] - Samples were spiked with high concentrations of Rat Ghrelin/Obestatin and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) [NBP2-82143] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Obestatin in samples. No Significant cross-reactivity or interference between Rat Obestatin and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 7%
Intra-Assay
CV% < 6%
Spike Recovery
average = 92% for serum; average = 92% for EDTA plasma; average = 100% for cell culture media
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHRL

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is a 28 residue pepetide expressed by stomach cells that is responsible for the hunger sensation. Expression is increased during times of fasting, and decreased after food consumption. Ghrelin is initially derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and decreased food intake. Ghrelin antibodies are useful tools for lipid and metabolism research as well as neuroscience studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat
Ghrelin/Obestatin ELI ...
NBP2-82143
Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

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Product PDFs

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  Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) - Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GHRL