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Ghrelin/Obestatin Antibody [Unconjugated]

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Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ghrelin/Obestatin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) followed by ...read more
Ghrelin/Obestatin was detected in immersion fixed frozen sections of mouse stomach using Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) at 10 µg/mL overnight at4 ...read more
Ghrelin/Obestatin was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Ghrelin/Obestatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8149) at 1 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF8149-SP
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Ghrelin/Obestatin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Ghrelin/Obestatin
Gly24-Lys117 (Leu72Met)
Accession # Q9UBU3
Specificity
Detects human Ghrelin/Obestatin in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse Ghrelin/Obestatin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GHRL
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ghrelin/Obestatin Antibody [Unconjugated]

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

GHRL (Ghrelin/Obestatin Prepropeptide) is a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98).  The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is modified with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ghrelin/Obestatin
Antibody [Unconjugate ...
AF8149
Ghrelin/Obestatin Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol GHRL
Uniprot