GDF-8/Myostatin Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, Neutralization, Western Blot.

Showing Publications 1 - 10 of 23. Show All 23 Publications. Collapse Publications.

Publications using AF788	Applications	Species
Yang, H;Wei, XS;Gong, J;Du, XM;Feng, HB;Su, C;Gilmore, C;Yue, C;Yu, SB;Li, C;Sui, HJ; The relationship between myodural bridge, atrophy and hyperplasia of the suboccipital musculature, and cerebrospinal fluid dynamics Scientific reports 2023-11-02 [PMID: 37919345] (Western Blot, Rat)	Western Blot	Rat
Bermejo-Álvarez, I;Pérez-Baos, S;Gratal, P;Medina, JP;Largo, R;Herrero-Beaumont, G;Mediero, A; Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia International journal of molecular sciences 2023-08-24 [PMID: 37685986] (Western Blot, Rabbit)	Western Blot	Rabbit
Guan, H;Yonemitsu, I;Ikeda, Y;Ono, T; Reversible Effects of Functional Mandibular Lateral Shift on Masticatory Muscles in Growing Rats Biomedicines 2023-07-27 [PMID: 37626623] (IHC, Rat)	IHC	Rat
Fengju Li, Ting Luo, Honghui Rong, Lu Lu, Ling Zhang, Chuanfeng Zheng et al. Maternal rodent exposure to di‐(2‐ethylhexyl) phthalate decreases muscle mass in the offspring by increasing myostatin Journal of Cachexia, Sarcopenia and Muscle 2022-12-01 [PMID: 36263449]
C Li, C Yue, ZC Liu, J Gong, XS Wei, H Yang, C Gilmore, SB Yu, GD Hack, HJ Sui The relationship between myodural bridges, hyperplasia of the suboccipital musculature, and intracranial pressure PLoS ONE, 2022-09-02;17(9):e0273193. 2022-09-02 [PMID: 36054096] (Western Blot, Rat)	Western Blot	Rat
Hong-Shi Li, Jiang-Ying Kuang, Gui-Jun Liu, Wei-Jie Wu, Xian-Lun Yin, Hao-Dong Li et al. Myostain is involved in ginsenoside Rb1-mediated anti-obesity Pharmaceutical Biology 2022-12-31 [PMID: 35639355]
H Muramatsu, T Kuramochi, H Katada, A Ueyama, Y Ruike, K Ohmine, M Shida-Kawa, R Miyano-Nis, Y Shimizu, M Okuda, Y Hori, M Hayashi, K Haraya, N Ban, T Nonaka, M Honda, H Kitamura, K Hattori, T Kitazawa, T Igawa, Y Kawabe, J Nezu Novel myostatin-specific antibody enhances muscle strength in muscle disease models Scientific Reports, 2021-01-25;11(1):2160. 2021-01-25 [PMID: 33495503] (Western Blot, Mouse)	Western Blot	Mouse
POtsch MS, Ishida J, Palus S et al. MT-102 prevents tissue wasting and improves survival in a rat model of severe cancer cachexia J Cachexia Sarcopenia Muscle 2/17/2020 [PMID: 32067370]
Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019-05-27;9(1):16. 2019-05-27 [PMID: 31133057] (Western Blot, Human)	Western Blot	Human
Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini Impact of heat therapy on recovery after eccentric exercise in humans Journal of Applied Physiology 2019-04-01 [PMID: 30605396]
Xingxing Kong, Ting Yao, Peng Zhou, Lawrence Kazak, Danielle Tenen, Anna Lyubetskaya et al. Brown Adipose Tissue Controls Skeletal Muscle Function via the Secretion of Myostatin Cell Metabolism 2018-10-01 [PMID: 30078553]
Parash Parajuli, Santosh Kumar, Audrey Loumaye, Purba Singh, Sailaja Eragamreddy, Thien Ly Nguyen et al. Twist1 Activation in Muscle Progenitor Cells Causes Muscle Loss Akin to Cancer Cachexia Developmental Cell 2018-06-01 [PMID: 29920276]
C Wallner, H Jaurich, JM Wagner, M Becerikli, K Harati, M Dadras, M Lehnhardt, B Behr Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2 Sci Rep, 2017-08-29;7(1):9878. 2017-08-29 [PMID: 28852138] (Neutralization, Mouse)	Neutralization	Mouse
RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Sci Rep, 2017-07-24;7(1):6311. 2017-07-24 [PMID: 28740214] (Western Blot, Rabbit)	Western Blot	Rabbit
J Ishida, M Konishi, M Saitoh, M Anker, SD Anker, J Springer Myostatin signaling is up-regulated in female patients with advanced heart failure Int. J. Cardiol., 2017-04-04;0(0):. 2017-04-04 [PMID: 28465115] (Western Blot, Human)	Western Blot	Human
O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-12-27;45(4):945-953. 2016-12-27 [PMID: 28027451] (Western Blot, Human)	Western Blot	Human
A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 2016-11-22;7(0):13363. 2016-11-22 [PMID: 27874859] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Jochen Springer, Susanne Schust, Katrin Peske, Anika Tschirner, Andre Rex, Odilo Engel et al. Catabolic signaling and muscle wasting after acute ischemic stroke in mice: indication for a stroke-specific sarcopenia Stroke 2014-12-01 [PMID: 25352483]
Potsch MS, Tschirner A, Palus S et al. The anabolic catabolic transforming agent (ACTA) espindolol increases muscle mass and decreases fat mass in old rats. J Cachexia Sarcopenia Muscle. 11/22/2013 [PMID: 24272787]
György Szláma, Mária Trexler, László Patthy Latent myostatin has significant activity and this activity is controlled more efficiently by WFIKKN 1 than by WFIKKN 2 The FEBS Journal 2013-08-01 [PMID: 23829672]
Moataz Elkasrawy, David Immel, Xuejun Wen, Xiaoyan Liu, Li-Fang Liang, Mark W. Hamrick Immunolocalization of Myostatin (GDF-8) Following Musculoskeletal Injury and the Effects of Exogenous Myostatin on Muscle and Bone Healing Journal of Histochemistry & Cytochemistry 2012-01-01 [PMID: 22205678]
Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am. J. Physiol., Cell Physiol., 2009-12-09;298(3):C465-76. 2009-12-09 [PMID: 20007454] (ICC, Rat)	ICC	Rat
Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JA Increased secretion and expression of myostatin in skeletal muscle from extremely obese women. Diabetes, 2008-10-03;58(1):30-8. 2008-10-03 [PMID: 18835929] (Western Blot, Human)	Western Blot	Human
Show All 23 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Zachary Patinkin	Simple Western	Human	06/23/2015	View Summary Application Simple Western Sample Tested Human Skeletal Muscle Lysate Species Human Lot EFM041506A
	5 reviewed by: Verified Customer	WB	Human	01/09/2015	View Summary Application Western Blot Sample Tested See PMID 23829672 Species Human
	4 reviewed by: Verified Customer	WB	Mouse	01/09/2015	View Summary Application Western Blot Sample Tested See PMID 23297411 Species Mouse

Array AF788

• GDF-8/Myostatin Antibodies
• GDF-8/Myostatin ELISA Kit
• GDF-8/Myostatin Lysate
• GDF-8/Myostatin ELISAs
• GDF-8/Myostatin Proteins and Enzymes