GDF-8/Myostatin Antibody - Propeptide

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using AF1539	Applications	Species
C Koch, S Buono, A Menuet, A Robé, S Djeddi, C Kretz, R Gomez-Oca, M Depla, A Monseur, L Thielemans, L Servais, J Laporte, BS Cowling Myostatin: a Circulating Biomarker Correlating with Disease in Myotubular Myopathy Mice and Patients Mol Ther Methods Clin Dev, 2020-05-04;17(0):1178-1189. 2020-05-04 [PMID: 32514412] (Western Blot, Mouse, Transgenic Mouse)	Western Blot	Mouse, Transgenic Mouse
M Pirruccell, J Jackson, S Wawersik, MT Webster, L Salta, K Long, W McConaughy, A Capili, C Boston, GJ Carven, NK Mahanthapp, KJ Turner, A Donovan Blocking extracellular activation of myostatin as a strategy for treating muscle wasting Sci Rep, 2018-02-02;8(1):2292. 2018-02-02 [PMID: 29396542] (Western Blot, Mouse)	Western Blot	Mouse
O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-12-27;45(4):945-953. 2016-12-27 [PMID: 28027451] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky T, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy A, Yancopoulos G, Gromada J, Stitt T Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice. Skelet Muscle, 2015-10-09;5(0):34. 2015-10-09 [PMID: 26457176] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
György Szláma, Mária Trexler, László Buday, László Patthy K153R polymorphism in myostatin gene increases the rate of promyostatin activation by furin FEBS Letters 1/30/2015 [PMID: 25543063]
Dubois V, Laurent M, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, Spans L, Gayan-Ramirez G, Deldicque L, Hespel P, Carmeliet G, Vanderschueren D, Claessens F A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle. FASEB J, 2014-03-26;28(7):2979-94. 2014-03-26 [PMID: 24671706] (Western Blot, Mouse)	Western Blot	Mouse
György Szláma, Mária Trexler, László Patthy Latent myostatin has significant activity and this activity is controlled more efficiently by WFIKKN 1 than by WFIKKN 2 The FEBS Journal 8/1/2013 [PMID: 23829672]
Lyons JA, Haring JS, Biga PR Myostatin expression, lymphocyte population, and potential cytokine production correlate with predisposition to high-fat diet induced obesity in mice. PLoS ONE, 2010-09-22;5(9):e12928. 2010-09-22 [PMID: 20877574] (Western Blot, Mouse)	Western Blot	Mouse
Lawrence T. Bish, Kevin J. Morine, Meg M. Sleeper, H. Lee Sweeney Myostatin Is Upregulated Following Stress in an Erk-Dependent Manner and Negatively Regulates Cardiomyocyte Growth in Culture and in a Mouse Model PLoS ONE 4/19/2010 [PMID: 20419100]
Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008-03-13;283(20):13874-88. 2008-03-13 [PMID: 18339631] (Western Blot, Human)	Western Blot	Human
Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition. J. Clin. Invest., 2006-10-12;116(11):2924-34. 2006-10-12 [PMID: 17039257] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	01/05/2015	View Summary Application Western Blot Sample Tested See PMID 23829672 Species Human

Array AF1539

• GDF-8/Myostatin Antibodies
• GDF-8/Myostatin ELISA Kit
• GDF-8/Myostatin Lysate
• GDF-8/Myostatin ELISAs
• GDF-8/Myostatin Proteins and Enzymes