![Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in transfected 293T cell line by GDF8 monoclonal antibody (M07), clone 3E7. Lane 1: GDF8 transfected lysatE (42.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GDF-8-Myostatin-Antibody-3E7-Western-Blot-H00002660-M07-img0004.jpg "Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in transfected 293T cell line by GDF8 monoclonal antibody (M07), clone 3E7. Lane 1: GDF8 transfected lysatE (42.8 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 3E7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | GDF8 (NP_005250, 241 a.a. ~ 310 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCS |
| Specificity | GDF8 (3E7) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MSTN |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GDF-8/Myostatin Antibody (H00002660-M07)Find related products by research area.
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![Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in LNCaP (Cat # L004V1).](http://images.novusbio.com/fullsize/GDF-8-Myostatin-Antibody-3E7-Western-Blot-H00002660-M07-img0003.jpg "Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in LNCaP (Cat # L004V1).")
![Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Myostatin expression in human bladder smooth muscle tissue & cells. (A) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder muscle tissue. Cell nuclei stained with DAPI (blue). 10× magnification (B) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder-derived hSMCs. 20× magnification (C) Flow cytometry comparison of myostatin-positive cells in pediatric bladder-derived control & ESLUTD hSMCs. (D) Representative immunoblot by WES of active myostatin (48 kDa) & its propeptides (100, 52 kDa) in bladder-derived smooth muscle tissue & cells. GAPDH (40 kDa) as a housekeeping protein. * p ≤ 0.05. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00002660-m07_mouse-monoclonal-gdf-8-myostatin-antibody-3e7-310202415345335.jpg "Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Myostatin expression in human bladder smooth muscle tissue & cells. (A) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder muscle tissue. Cell nuclei stained with DAPI (blue). 10× magnification (B) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder-derived hSMCs. 20× magnification (C) Flow cytometry comparison of myostatin-positive cells in pediatric bladder-derived control & ESLUTD hSMCs. (D) Representative immunoblot by WES of active myostatin (48 kDa) & its propeptides (100, 52 kDa) in bladder-derived smooth muscle tissue & cells. GAPDH (40 kDa) as a housekeeping protein. * p ≤ 0.05. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Myostatin expression in human bladder smooth muscle tissue & cells. (A) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder muscle tissue. Cell nuclei stained with DAPI (blue). 10× magnification (B) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder-derived hSMCs. 20× magnification (C) Flow cytometry comparison of myostatin-positive cells in pediatric bladder-derived control & ESLUTD hSMCs. (D) Representative immunoblot by WES of active myostatin (48 kDa) & its propeptides (100, 52 kDa) in bladder-derived smooth muscle tissue & cells. GAPDH (40 kDa) as a housekeeping protein. * p ≤ 0.05. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00002660-m07_mouse-monoclonal-gdf-8-myostatin-antibody-3e7-31020241539250.jpg "Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Myostatin expression in human bladder smooth muscle tissue & cells. (A) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder muscle tissue. Cell nuclei stained with DAPI (blue). 10× magnification (B) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder-derived hSMCs. 20× magnification (C) Flow cytometry comparison of myostatin-positive cells in pediatric bladder-derived control & ESLUTD hSMCs. (D) Representative immunoblot by WES of active myostatin (48 kDa) & its propeptides (100, 52 kDa) in bladder-derived smooth muscle tissue & cells. GAPDH (40 kDa) as a housekeeping protein. * p ≤ 0.05. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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