![Western Blot: GCKR Antibody (1E7) [NBP2-03396] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-GCKR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/GCKR-Antibody-1E7-Western-Blot-NBP2-03396-img0006.jpg "Western Blot: GCKR Antibody (1E7) [NBP2-03396] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-GCKR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI1E7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human GCKR (NP_001417) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GCKR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GCKR Antibody (NBP2-03396)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: GCKR Antibody (1E7) [NBP2-03396] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR.](http://images.novusbio.com/fullsize/GCKR-Antibody-1E7-Immunocytochemistry-Immunofluorescence-NBP2-03396-img0004.jpg "Immunocytochemistry/Immunofluorescence: GCKR Antibody (1E7) [NBP2-03396] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR.")
![Immunohistochemistry-Paraffin: GCKR Antibody (1E7) [NBP2-03396] - Staining of paraffin-embedded Human Kidney tissue using anti-GCKR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GCKR-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-03396-img0003.jpg "Immunohistochemistry-Paraffin: GCKR Antibody (1E7) [NBP2-03396] - Staining of paraffin-embedded Human Kidney tissue using anti-GCKR mouse monoclonal antibody.")
![Western Blot: GCKR Antibody (1E7) [NBP2-03396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCKR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCKR.](http://images.novusbio.com/fullsize/GCKR-Antibody-1E7-Western-Blot-NBP2-03396-img0001.jpg "Western Blot: GCKR Antibody (1E7) [NBP2-03396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCKR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCKR.")
![Immunohistochemistry-Paraffin: GCKR Antibody (1E7) [NBP2-03396] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCKR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GCKR-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-03396-img0002.jpg "Immunohistochemistry-Paraffin: GCKR Antibody (1E7) [NBP2-03396] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCKR mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")