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Melatonin Receptor 1B Antibody - BSA Free

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Genetic Strategies: Knockdown Validated: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Melatonin Receptor 1B Antibody [NLS932] - Analysis for collagen I, fibronectin, and Alpha-smooth muscle actin ...read more
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Melatonin receptor type 1 B (MTR1B) in rat tissues fixed in paraformaldehyde and embedded in paraffin. A,B-brain, C-prostate. Image from verified ...read more
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term diabetic treated with MLT (MD1) Influence ...read more
Western Blot: Melatonin Receptor 1B Antibody [NLS932] - Analysis of 1) HepG2 and 2) A431.
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term control (C1). Influence of Melatonin on the ...read more
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain. 8 ug/ ml.
Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Analysis of Human Brain using Fast Red with hematoxylin counterstain.
Immunohistochemistry: Melatonin Receptor 1B Antibody [NLS932] - Staining of Mouse Brain.
Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. Short-term control treated with MLT (M1). Influence of ...read more
Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control ...read more
Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control ...read more
Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control ...read more
Western Blot: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Effects of luzindole on inhibitory action of melatonin on TGF-beta 1-stimulated proliferation & activation in renal interstitial fibroblasts. (A) ...read more
Immunohistochemistry: Melatonin Receptor 1B Antibody - BSA Free [NLS932] - Tissue localization of melatonin receptor type 1B (MTR1B) in the ventral prostate of rats. (a) Short-term control (C1); (b) short-term control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

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Melatonin Receptor 1B Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the human Melatonin Receptor 1B protein (within residues 200-300) [Swiss-Prot P49286].
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTNR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24365461)
  • Immunohistochemistry-Paraffin 1:100
  • Knockdown Validated
  • Western Blot 1:1000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control Peptide
Melatonin Receptor 1B Protein (NLS932PEP)
Reviewed Applications
Read 1 Review rated 4
using
NLS932 in the following applications:

Publications
Read Publications using
NLS932 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID:32758663).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Melatonin Receptor 1B Antibody - BSA Free

  • FGQTL2
  • Mel 1b melatonin receptor
  • Mel1b receptor
  • MEL-1B-R
  • melatonin receptor 1B
  • melatonin receptor MEL1B
  • melatonin receptor type 1B
  • MT2

Background

Melatonin receptor type 1B (MTNR1B) is a member of the G-protein coupled receptor 1 family. This family is responsible for translating external chemical signals across the cell membrane. MTNR1B is one of two high affinity receptors for melatonin, a hormone secreted by the pineal gland. Pertussis toxin sensitive G proteins, that inhibit adenylate cyclase activity, control the MTNR1B receptor. MTNR1B is expressed in the retina, and to a lesser extent the brain and hippocampus. MTNR1B is thought to regulate reproduction, the circadian cycle, and neurological melatonin levels; as well as participate in light dependent function in the retina. Post translational modifications that may affect MTNR1B are disulfide bonds and glycosylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melatonin Receptor 1B Antibody (NLS932)(10)

We have publications tested in 3 confirmed species: Mouse, Rat, Chicken.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.


Filter By Application
IHC-Fr
(1)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Mouse
(2)
Rat
(3)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NLS932 Applications Species
Yan L, Han X, Zhang M et al. Melatonin exerts neuroprotective effects in mice with spinal cord injury by activating the Nrf2/Keap1 signaling pathway via the MT2 receptor Experimental and Therapeutic Medicine 2023-11-28 [PMID: 38125360] (WB, Mouse) WB Mouse
Wu D, Zhao D, Huang D et al. Estrogen-dependent depressor response of melatonin via baroreflex afferent function and intensification of PKC-mediated Nav1.9 activation Acta pharmacologica Sinica 2022-02-07 [PMID: 35132193] (WB, Rat) WB Rat
Xu G, Yuan Z, Hou J Et al. Prolonging photoperiod promotes testosterone synthesis of Leydig cells by directly targeting local melatonin system in rooster testes Biology of reproduction 2021-08-17 [PMID: 34401899]
Mikyung Kim, So Min Lee, Jeeyoun Jung, Yun Jin Kim, Kyo Chul Moon, Ji Hae Seo, Tae Kyung Ha, Eunyoung Ha Pinealectomy increases thermogenesis and decreases lipogenesis Molecular Medicine Reports 2020-11-01 [PMID: 33000192]
Dong Y, Zhao J, Zhu Q et al. Melatonin inhibits the apoptosis of rooster Leydig cells by suppressing oxidative stress via AKT-Nrf2 pathway activation Free Radic. Biol. Med. 2020-08-03 [PMID: 32758663] (WB, Chicken) WB Chicken
Kim JY, Park JH, Jeon EJ et al. Melatonin Prevents Transforming Growth Factor-beta1-Stimulated Transdifferentiation of Renal Interstitial Fibroblasts to Myofibroblasts by Suppressing Reactive Oxygen Species-Dependent Mechanisms Antioxidants (Basel) 2020-01-01 [PMID: 31906396] (WB, Rat) WB Rat
Takumida M, Anniko M Localization of melatonin and its receptors (melatonin 1a and 1b receptors) in the mouse inner ear Acta Otolaryngol. 2019-11-01 [PMID: 31561736]
Shuntai Yu, Xuefeng Zhang, Zilan Xu, Changlong Hu Melatonin promotes proliferation of neural stem cells from adult mouse spinal cord via the PI3K/AKT signaling pathway. FEBS letters 2020-06-15 [PMID: 31127855]
Gobbo MG, Dizeyi N, Abrahamsson PA et al. Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions. J Diabetes Res. 2015-08-21 [PMID: 26295055] (IHC-P, Rat)

Details:
Melatonin Receptor 1B antibody was used for IHC-P analysis of ventral prostate tissues from normal or hyperglycemic rats that were subjected to Melatonin treatments. The primary antibody was used at 1?:?75 dilution with overnight 4C incubation and detection involved HRP-diaminobenzidine/DAB based detection (Figure 5).
IHC-P Rat
Corthell JT, Olcese J, Trombley PQ. Melatonin in the mammalian olfactory bulb. Neuroscience 2013-12-21 [PMID: 24365461] (IHC-Fr, WB, Mouse) IHC-Fr, WB Mouse

Review for Melatonin Receptor 1B Antibody (NLS932) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NLS932:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Melatonin Receptor 1B NLS932
Enlarge
4
reviewed by:
Verified Customer
IHC-P Rat 09/03/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat ventral prostate and brain.
SpeciesRat
LotA-1

Product General Protocols

View specific protocols for Melatonin Receptor 1B Antibody (NLS932): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Melatonin Receptor 1B Antibody (NLS932). (Showing 1 - 2 of 2 FAQs).

  1. I saw that you have the antibodies for the both receptors of melatonin but not listed with FACS application. I need more information about what is better for my assay.
    • At this time, none of our melatonin receptor antibodies have been tested and validated in Flow. Should you choose to purchase and test one of them, you would qualify for the Innovator's Rewards program. You should take a look at each individual antibody, the clonality, conjugates, immunogen region and all relevant data in order to decide which product would be most suitable for your needs.
  2. Would you let me know the tissue for positive control of NLS932?
    • This antibody was tested in both mouse brain and human brain. These are our recommended positive controls. According to UniProt, this protein is also present in retina and hippocampus (UniProt P49286) so these may also be suitable.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
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1
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Verified Customer
09/03/2014
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol MTNR1B
Entrez
Uniprot