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MAP4K2 Antibody

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Immunocytochemistry/Immunofluorescence: MAP4K2 Antibody [NBP1-88584] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunocytochemistry/ Immunofluorescence: MAP4K2 Antibody [NBP1-88584] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining in human lymph node and liver tissues using anti-MAP4K2 antibody. Corresponding MAP4K2 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining of human lymph node shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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MAP4K2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEPLSSPPGTLPPPPSGPNSSPLLPTAWATMKQRE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP4K2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAP4K2 Protein (NBP1-88584PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAP4K2 Antibody

  • B lymphocyte serine/threonine protein kinase
  • B lymphocyte serine/threonine-protein kinase
  • BL44
  • EC 2.7.11
  • EC 2.7.11.1
  • GC kinase
  • GCKMEK kinase kinase 2
  • Germinal center kinase
  • germinal centre kinase (GC kinase)
  • MAP4K2
  • MAPK/ERK kinase kinase kinase 2
  • MEK kinase kinase 2
  • MEKKK 2
  • mitogen-activated protein kinase kinase kinase kinase 2
  • Rab8 interacting protein
  • Rab8-interacting protein
  • RAB8IP
  • RAB8IPMEKKK 2

Background

MAP4K2 is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP4K2