Novus Biologicals products are now on bio-techne.com

GATA-3 Antibody (634913) [Unconjugated]

Images

 
GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer using Mouse Anti-Human GATA‑3 Monoclonal Antibody (MAB6330) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF Membrane ...read more
GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 3 µg/mL for 3 hours at room temperature. Cells were stained ...read more
GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 15 µg/mL overnight at 4 °C. Before ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for GATA‑3 at approximately 53 ...read more
Orthogonal Strategies: GATA-3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 403551) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, Dual ISH-IHC, ICC/IF, mIF
Clone
634913
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

GATA-3 Antibody (634913) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Specificity
Detects human and mouse GATA-3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GATA3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB6330 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody (634913) [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

6507-IL/CF
Species: Hu
Applications: BA
MAB5385
Species: Hu
Applications: ICC, ICFlow, WB
AF2605
Species: Hu
Applications: ICC, IHC, Simple Western, WB
M5000
Species: Mu
Applications: ELISA
DY413
Species: Mu
Applications: ELISA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DY417
Species: Mu
Applications: ELISA
NBP2-25241
Species: Hu, Mu
Applications: ICC/IF, IP, WB
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
AF2046
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
NBP1-87992
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
202-IL
Species: Hu
Applications: BA
DKK300
Species: Hu
Applications: ELISA
NBP1-47492
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NB600-922
Species: Ch
Applications: ELISA, IHC, IP, Single-Cell Western, WB

Publications for GATA-3 Antibody (MAB6330)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(3)
IHC-P
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MAB6330 Applications Species
Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K; Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s Cell reports 2023-06-07 [PMID: 37294636] (Western Blot, Human) Western Blot Human
Song-Chang Lin, Guoyu Yu, Yu-Chen Lee, Jian H. Song, Xingzhi Song, Jianhua Zhang et al. Endothelial-to-osteoblast transition in normal mouse bone development iScience 2023-02-17 [PMID: 36798441]
Ruan D, Ye Z, Yuan S et al. Human Early Syncytiotrophoblasts Are Highly Susceptible to SARS-CoV-2 Infection Cell Reports Medicine 11/1/2022 [PMID: 36495872]
F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 2022-10-01;21(12):103207. 2022-10-01 [PMID: 36191778] (Western Blot, Human) Western Blot Human
Peter T Ruane, Terence Garner, Lydia Parsons, Phoebe A Babbington, Ivan Wangsaputra, Susan J Kimber et al. Trophectoderm differentiation to invasive syncytiotrophoblast is promoted by endometrial epithelial cells during human embryo implantation Human Reproduction 2022-04-01 [PMID: 35079788]
Y Li, S Yu, C Huang, L Diao, C Chen, W Liu, R Lian, M Mo, C Du, F Liu, Y Zeng Predictive role of endometrial T-bet/GATA3 ratio during mid-luteal phase for live birth in patients undergoing in vitro fertilization: A retrospective observational study Journal of reproductive immunology, 2021-12-16;149(0):103465. 2021-12-16 [PMID: 34953324] (IHC, Human) IHC Human
Y Fan, Z Min, S Alsolami, Z Ma, E Zhang, W Chen, K Zhong, W Pei, X Kang, P Zhang, Y Wang, Y Zhang, L Zhan, H Zhu, C An, R Li, J Qiao, T Tan, M Li, Y Yu Generation of human blastocyst-like structures from pluripotent stem cells Cell Discovery, 2021-09-07;7(1):81. 2021-09-07 [PMID: 34489415] (IHC, Human) IHC Human
Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury iScience 2021-08-20 [PMID: 34381965]
Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer Breast Cancer Research 2020-12-09 [PMID: 33298139]
KT Minn, YC Fu, S He, S Dietmann, SC George, MA Anastasio, SA Morris, L Solnica-Kr High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures Elife, 2020-11-18;9(0):. 11/18/2020 [PMID: 33206048]
Show All 16 Publications.

Reviews for GATA-3 Antibody (MAB6330) (0)

There are no reviews for GATA-3 Antibody (MAB6330). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-3 Antibody (MAB6330) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GATA-3 Products

Blogs on GATA-3.


  Read full blog post.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GATA-3 Antibody (634913) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GATA3
Uniprot