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GATA-3 Antibody (291106) [Unconjugated]

 (5 Publications)
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Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG ...read more
GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA‑3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
291106
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Phycoerythrin
IC26051P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC26051P
Carrier Free
MAB26051-SP
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GATA-3 Antibody (291106) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GATA3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB26051 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody (291106) [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GATA-3 Antibody
(291106) [Unconjugate
MAB26051
GATA-3 Antibody (291106) [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for GATA-3 Antibody (MAB26051)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, Immunoprecipitation.


Filter By Application
IHC-P
(1)
Immunoprecipitation
(1)
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Human
(1)
Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using MAB26051 Applications Species
M Fujimoto, M Yokoyama, M Kiuchi, H Hosokawa, A Nakayama, N Hashimoto, I Sakuma, H Nagano, K Yamagata, F Kudo, I Manabe, E Lee, R Hatano, A Onodera, K Hirahara, K Yokote, T Miki, T Nakayama, T Tanaka Liver group 2 innate lymphoid cells regulate blood glucose levels through IL-13 signaling and suppression of gluconeogenesis Nature Communications, 2022-09-15;13(1):5408. 2022-09-15 [PMID: 36109558] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Hiroyuki Hosokawa, Maria Koizumi, Kaori Masuhara, Maile Romero-Wolf, Tomoaki Tanaka, Toshinori Nakayama et al. Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro–T cells Journal of Experimental Medicine 2021-08-02 [PMID: 34180951]
Hiroyuki Hosokawa, Maile Romero-Wolf, Qi Yang, Yasutaka Motomura, Ditsa Levanon, Yoram Groner et al. Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells Journal of Experimental Medicine 2020-01-06 [PMID: 31653691]
Hiroyuki Hosokawa, Jonas Ungerbäck, Xun Wang, Masaki Matsumoto, Keiichi I. Nakayama, Sarah M. Cohen et al. Transcription Factor PU.1 Represses and Activates Gene Expression in Early T Cells by Redirecting Partner Transcription Factor Binding Immunity 2018-06-11 [PMID: 29924977]
Inman D, Kawana K, Schust D Cyclic regulation of T-Bet and GATA-3 in human endometrium. Reprod Sci, 2008-01-01;15(1):83-90. 2008-01-01 [PMID: 18212358] (IHC-P, Human) IHC-P Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA3
Uniprot