![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Western-Blot-NBP3-03282-img0004.jpg "Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human Gastrin-releasing Peptide/Bombesin/Neuromedin C (NP_002082.2). MRGRELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSEDSSNFKDVGSKGKVGRLSAPGSQREGRNPQLNQQ |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GRP |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody (NBP3-03282)Find related products by research area.
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| Gene Symbol | GRP |
![Immunocytochemistry/Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Immunofluorescence analysis of HeLa cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03282-img0001.jpg "Immunocytochemistry/Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Immunofluorescence analysis of HeLa cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0003.jpg "Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing Peptide-Bombesin-Neuromedin C Antibody-Western Blot-NBP3-03282-img0005.jpg "Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0002.jpg "Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
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