Galectin-3 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: ELISA Capture, ELISA Development (Detection), Flow Cytometry, ICC, IHC, IHC-P, Western Blot.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using AF1154	Applications	Species
Namrita Kaur, Laura Rodriguez de la Ballina, Håvard Styrkestad Haukaas, Maria Lyngaas Torgersen, Maja Radulovic, Michael J Munson et al. TECPR1 is activated by damage‐induced sphingomyelin exposure to mediate noncanonical autophagy The EMBO Journal 9/4/2023 [PMID: 37409525]
D Zheng, J Zhou, L Qian, X Liu, C Chang, S Tang, H Zhang, S Zhou Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment Bioactive materials, 2022-11-03;22(0):567-587. 2022-11-03 [PMID: 36382024] (Western Blot, Human)	Western Blot	Human
Du H, Wong MY, Zhang T et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses Life science alliance 7/1/2021 [PMID: 34103390]
Kirstin Faust, Nancy Freitag, Gabriela Barrientos, Christoph Hartel, Sandra M. Blois Galectin-Levels Are Elevated in Infants Born Preterm Due to Amniotic Infection and Rapidly Decline in the Neonatal Period Frontiers in Immunology 2/25/2021 [PMID: 33717050]
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020-10-07;8(1):163. 2020-10-07 [PMID: 33028409] (Western Blot, Human)	Western Blot	Human
Maja Krstić Ristivojević, Jeanette Grundström, Danijela Apostolović, Mirjana Radomirović, Vesna Jovanović, Vlad Radoi et al. Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer International Journal of Molecular Sciences 8/11/2020 [PMID: 32796496]
N Freitag, I Tirado-Gon, G Barrientos, KL Powell, P Boehm-Stur, SP Koch, K Hecher, AC Staff, PC Arck, A Diemert, SM Blois Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency Cell Death Dis, 2020-07-23;11(7):560. 2020-07-23 [PMID: 32703931] (ELISA Capture, Human)	ELISA Capture	Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 4/2/2020 [PMID: 32243809]
Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac Human trophoblast requires galectin-3 for cell migration and invasion Sci Rep, 2019-02-14;9(1):2136. 2019-02-14 [PMID: 30765738] (ELISA Development (Detection), Flow Cytometry, ICC, Human)	ELISA Development (Detection), Flow Cytometry, ICC	Human
Maja Radulovic, Kay O Schink, Eva M Wenzel, Viola Nähse, Antonino Bongiovanni, Frank Lafont et al. ESCRT ‐mediated lysosome repair precedes lysophagy and promotes cell survival The EMBO Journal 11/2/2018 [PMID: 30314966]
T Shimura, Y Kofunato, R Okada, R Yashima, Y Koyama, K Araki, H Kuwano, S Takenoshit Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma Oncol Lett, 2017-05-25;14(1):819-829. 2017-05-25 [PMID: 28693238] (IHC, Human)	IHC	Human
JIA LIU, YULING LIU, WULIANG WANG, CHENYANG WANG, YANHONG CHE Expression of immune checkpoint molecules in endometrial carcinoma Experimental and Therapeutic Medicine 11/1/2015 [PMID: 26640578]
Maryam Salimi, Luzheng Xue, Helen Jolin, Clare Hardman, David J. Cousins, Andrew N. J. McKenzie et al. Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production1 The Journal of Immunology 1/1/2016 [PMID: 26582946]
Fulwah Alqahtani, Jafar Mahdavi, Lee M. Wheldon, Matthew Vassey, Necmettin Pirinccioglu, Pierre-Joseph Royer et al. Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis Open Biology 10/3/2014 [PMID: 25274119]
Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury. Exp Cell Res, 2014-06-19;326(1):143-54. 2014-06-19 [PMID: 24954408] (Western Blot, Human)	Western Blot	Human
Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8. FASEB J., 2010-09-27;25(1):242-54. 2010-09-27 [PMID: 20876211] (Flow Cytometry, ICC, Western Blot, Human)	Flow Cytometry, ICC, Western Blot	Human
Zhuo Y, Chammas R, Bellis SL Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis. J. Biol. Chem., 2008-08-08;283(32):22177-85. 2008-08-08 [PMID: 18676377] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Thijssen VL, Hulsmans S, Griffioen AW The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells. Am. J. Pathol., 2008-01-17;172(2):545-53. 2008-01-17 [PMID: 18202194] (Flow Cytometry, ICC, IHC-P, Western Blot, Human)	Flow Cytometry, ICC, IHC-P, Western Blot	Human
Show All 18 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Michela Colombo	IMC	Human	11/12/2021	View Summary Application Imaging Mass Cytometry Sample Tested bone marrow Species Human Lot WZE0418091 Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.***
	4 reviewed by: Verified Customer	IF	Human	01/05/2015	View Summary Application Immunofluorescence Sample Tested See PMID 23121677 Species Human

Array AF1154

• Galectin-3 Antibodies
• Galectin-3 Antibody Pair
• Galectin-3 ELISA Kits
• Galectin-3 Lysate
• Galectin-3 Proteins
• Galectin-3 ELISAs
• Galectin-3 Proteins and Enzymes