Galectin-3 Antibodies
We offer Galectin-3 Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Intracellular), Flow Cytometry, Functional, Immunoassay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Sandwich ELISA, Simple Western, Western Blot. Each Galectin-3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Galectin-3 Antibodies can be used in a variety of model species: Human, Mouse, Rabbit, Rat. Use the list below to choose the Galectin-3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Galectin-3 polyclonal antibodies and browse our Galectin-3 monoclonal antibody catalog.
Alternate Names for Galectin-3 Antibodies
anti-Galectin-3 antibody, anti-LGALS3 antibody, anti-Carbohydrate-binding protein 35 antibody, anti-CBP 35,35 kDa lectin antibody, anti-Gal-3 antibody, anti-galactin-3 antibody, anti-Galactose-specific lectin 3 antibody, anti-Galactoside-binding protein antibody, anti-GALBPCBP35 antibody, anti-GALIG antibody, anti-IgE-binding protein antibody, anti-L-31 antibody, anti-L31 antibody, anti-Laminin-binding protein antibody, anti-Lectin L-29 antibody, anti-lectin, galactoside-binding, soluble, 3 antibody, anti-LGALS2 antibody, anti-MAC-2 antibody, anti-Mac-2 antigen antibody, anti-MAC2GAL3 antibody
23 Results for "galectin-3" in Primary Antibodies
![Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Western-Blot-NB300-538-img0017.jpg "Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0019.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0016.jpg)
![Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Flow-Cytometry-NB300-538-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of rat olfactory bulb](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of HeLa cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0013.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0014.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0015.jpg)
![Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Western-Blot-NBP1-92690-img0002.jpg "Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - MDA-MB-231 cells stained for Gal-3 (red, arrows), shown with DAPI counterstained nuclei (blue). Negative control received non-specific antibody instead of primary antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to galectin-3, NBP1-92690, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The NBP1-92690 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while the MAP2 antibody labels dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Galectin-3 protein stained by Galectin-3 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16589-img0005.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - HBL435 xenograft, using Galectin3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16589-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0006.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16589-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16589_rabbit-polyclonal-galectin-3-antibody-1610202419414598.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Western Blot: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-3.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Western-Blot-NBP2-45618-img0022.jpg "Western Blot: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-3.")
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0021.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0001.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0002.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0003.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0004.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0005.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0006.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0007.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0008.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0009.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0010.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0011.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0012.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0013.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0014.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0015.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0016.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0017.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0018.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0019.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0020.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0005.jpg "Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.")
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - An intracellular stain was performed on RAW 246.7 cells with Galectin-3 Antibody (M3/38) NBP1-43313AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0003.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody [NBP3-03252] - Analysis of U-2 OS cells using Galectin-3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03252-img0003.jpg "Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody [NBP3-03252] - Analysis of U-2 OS cells using Galectin-3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody [NBP3-03252] - Analysis of C6 cells using Galectin-3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03252-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody [NBP3-03252] - Analysis of L-929 cells using Galectin-3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03252-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Knockout-Validated-NBP3-03252-img0004.jpg)