HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # ...read more
HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed ...read more
Detects mouse Frizzled-1 in
direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry.
In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse
(rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh)
Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FZD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Frizzled-1 Antibody (162531) [Unconjugated]
DKFZp564G072
FLJ95923
frizzled family receptor 1
frizzled homolog 1
frizzled, Drosophila, homolog of, 1
Frizzled1
Frizzled-1
fz-1
FZD1
fzE1
hFz1
seven transmembrane spanning receptor
Wnt receptor
Background
The
Wnt genes encode a large family of glycoproteins that are essential in
development and tissue maintenance (1). Members of the Frizzled family
of proteins serve as receptors for the Wnt signaling pathway (2). Ten
mouse and human Frizzled genes have been identified to date. The
predicted structure of Frizzled proteins is similar among all family
members, containing a divergent N-terminal signal peptide, a highly
conserved extracellular cysteine-rich domain (CRD), a variable-length
linker region, a seven-pass transmembrane region, and a variable-length
C-terminal cytoplasmic domain. The CRD comprises 642 amino acids
and shares 95% identity with the human orthologue. Frizzled-1
mRNA has been detected in relatively large amounts in adult heart,
placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung
and kidney (3). Several Frizzled-dependent signaling pathways exist
(2). Their activation depends on the Wnt ligand and the cell context.
Members of the low density lipoprotein receptor-related protein (LRP)
are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in
the Wnt/Frizzled canonical pathway that alters gene expression via the
stabilization of beta -catenin (4, 5). LRP1 may down regulate
Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering
Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors
whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1
may be part of a feedback mechanism to modulate the effects of BMP-2 in
mesenchymal cells since upregulation of its expression by BMP-2
counteracts the effects of BMP-2 and Wnt-3a in inducing the expression
of the osteoblast differentiation marker, alkaline phosphatase (8).
Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
Hsieh, J-C. (2004) Front. Biosci., 9:1333.
Xu, L. et al. (2001) Matrix Biol. 2:147.
Tamai, K. et al. (2000) Nature 407:530.
Pinson, K. et al. (2000) Nature 407:535.
Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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