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Frizzled-1 Antibody (162531) [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
162531
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
LYOPH

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Frizzled-1 Antibody (162531) [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421
Specificity
Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FZD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Readout System
Publications
Read Publications using
BAM11201 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-1 Antibody (162531) [Biotin]

  • DKFZp564G072
  • FLJ95923
  • frizzled family receptor 1
  • frizzled homolog 1
  • frizzled, Drosophila, homolog of, 1
  • Frizzled1
  • Frizzled-1
  • fz-1
  • FZD1
  • fzE1
  • hFz1
  • seven transmembrane spanning receptor
  • Wnt receptor

Background

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-1 Antibody (BAM11201)(3)

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Bioinformatics

Gene Symbol FZD1
Uniprot