Frizzled-1 Antibody (162531) [Alexa Fluor® 750] Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1 Gln72-His248 (Met122Ile) Accession # O70421 |
Specificity |
Detects human and mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
FZD1 |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative |
0.09% Sodium Azide |
Concentration |
Please see the vial label for concentration. If unlisted please contact technical services. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Frizzled-1 Antibody (162531) [Alexa Fluor® 750]
Background
The
Wnt genes encode a large family of glycoproteins that are essential in
development and tissue maintenance (1). Members of the Frizzled family
of proteins serve as receptors for the Wnt signaling pathway (2). Ten
mouse and human Frizzled genes have been identified to date. The
predicted structure of Frizzled proteins is similar among all family
members, containing a divergent N-terminal signal peptide, a highly
conserved extracellular cysteine-rich domain (CRD), a variable-length
linker region, a seven-pass transmembrane region, and a variable-length
C-terminal cytoplasmic domain. The CRD comprises 642 amino acids
and shares 95% identity with the human orthologue. Frizzled-1
mRNA has been detected in relatively large amounts in adult heart,
placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung
and kidney (3). Several Frizzled-dependent signaling pathways exist
(2). Their activation depends on the Wnt ligand and the cell context.
Members of the low density lipoprotein receptor-related protein (LRP)
are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in
the Wnt/Frizzled canonical pathway that alters gene expression via the
stabilization of beta -catenin (4, 5). LRP1 may down regulate
Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering
Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors
whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1
may be part of a feedback mechanism to modulate the effects of BMP-2 in
mesenchymal cells since upregulation of its expression by BMP-2
counteracts the effects of BMP-2 and Wnt-3a in inducing the expression
of the osteoblast differentiation marker, alkaline phosphatase (8).
- Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
- Hsieh, J-C. (2004) Front. Biosci., 9:1333.
- Xu, L. et al. (2001) Matrix Biol. 2:147.
- Tamai, K. et al. (2000) Nature 407:530.
- Pinson, K. et al. (2000) Nature 407:535.
- Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
- Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
- Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, WB
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: BA, BA
Species: Hu
Applications: CyTOF-ready, Flow, ICC
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: WB
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: BA
Species: Mu
Applications: IHC, WB
Species: Hu, Mu, Pm
Applications: WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Mu
Applications: IHC
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: WB
Publications for Frizzled-1 Antibody (FAB11201S) (0)
There are no publications for Frizzled-1 Antibody (FAB11201S).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Frizzled-1 Antibody (FAB11201S) (0)
There are no reviews for Frizzled-1 Antibody (FAB11201S).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Frizzled-1 Antibody (FAB11201S) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional Frizzled-1 Products
Blogs on Frizzled-1