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Filamin A Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Analysis in human prostate and skeletal muscle tissues. Corresponding FLNA RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human kidney, liver, pancreas and testis using Anti-FLNA antibody NBP1-90283 (A) shows similar protein ...read more
Independent Antibodies: Western Blot: Filamin A Antibody [NBP1-90283] - Analysis using Anti-FLNA antibody NBP1-90283 (A) shows similar pattern to independent antibody NBP1-90284 (B).
Western Blot: Filamin A Antibody [NBP1-90283] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis using Anti-FLNA antibody NBP1-90283.
Western Blot: Filamin A Antibody [NBP1-90283] - Analysis in human cell line U-251 MG.
Western Blot: Filamin A Antibody [NBP1-90283] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human endometrium shows strong cytoplasmic positivity in stromal cells.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis shows moderate cytoplasmic positivity in peritubular myoid cells.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human kidney using Anti-FLNA antibody.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human pancreas using Anti-FLNA antibody.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human liver using Anti-FLNA antibody.
Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Filamin A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Filamin A Protein (NBP1-90283PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Filamin A Antibody

  • ABP-280
  • ABP-280DKFZp434P031
  • ABPX
  • actin binding protein 280
  • Actin-binding protein 280
  • Alpha-filamin
  • CVD1
  • EC 2.1.1.43
  • EC 6.3.4.4
  • Endothelial actin-binding protein
  • Filamin A
  • filamin A, alpha (actin binding protein 280)
  • filamin A, alpha
  • Filamin-1
  • filamin-A
  • FLN
  • FLN1
  • FLN1OPD
  • FLNA
  • FLN-A
  • FMD
  • MNS
  • NHBP
  • Non-muscle filamin
  • OPD1
  • OPD2
  • XLVD
  • XMVD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Filamin A Antibody (NBP1-90283). (Showing 1 - 1 of 1 FAQ).

  1. May I know if I should use this antibody (NBP1-90283) with milk or BSA?
    • When using NBP1-90283 for Western blot, you should use milk to block. For IHC, you should use serum from the host of the secondary antibody.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLNA