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Filamin A Antibody

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Western Blot: Filamin A Antibody [NB100-58814] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58814. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody NB100-58813.
Independent Antibodies: Western Blot: Filamin A Antibody [NB100-58814] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58814. Filamin A was also immunoprecipitated by rabbit ...read more
Immunohistochemistry-Paraffin: Filamin A Antibody [NB100-58814] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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Filamin A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2597 and 2647 of human Filamin A using the numbering given in entry NP_001447.1 (GeneID 2316).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLNA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Filamin A Antibody

  • ABP-280
  • ABP-280DKFZp434P031
  • ABPX
  • actin binding protein 280
  • Actin-binding protein 280
  • Alpha-filamin
  • CVD1
  • EC 2.1.1.43
  • EC 6.3.4.4
  • Endothelial actin-binding protein
  • Filamin A
  • filamin A, alpha (actin binding protein 280)
  • filamin A, alpha
  • Filamin-1
  • filamin-A
  • FLN
  • FLN1
  • FLN1OPD
  • FLNA
  • FLN-A
  • FMD
  • MNS
  • NHBP
  • Non-muscle filamin
  • OPD1
  • OPD2
  • XLVD
  • XMVD

Background

Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FLNA
Entrez
Uniprot