Reactivity | HuSpecies Glossary |
Applications | WB |
Clone | 36923 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9 Met1-Ser208 Accession # P31371 |
Specificity | Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -7, -10, -11, -13, -16, -17, -18, -19, rhFGF acidic, rhFGF basic, recombinant mouse (rm) FGF‑6, -8b, -8c, or -15 is observed. In Western blots, no cross-reactivity with rhFGF-3, -4, -5, -6, -7, -10, -13, rmFGF-8b, or rmFGF-8c is observed. |
Source | N/A |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | FGF9 |
Purity Statement | Protein A or G purified from ascites |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30‑50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.
The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.
Secondary Antibodies |
Isotype Controls |
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