Novus Biologicals products are now on bio-techne.com

FEN-1 Antibody (OTI1F3) - Azide and BSA Free

Images

 
Western Blot: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN-1.
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of A549 cell lysate (35ug).
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

FEN-1 Antibody (OTI1F3) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 117-380 of human FEN1 (NP_004102) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FEN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
42.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FEN-1 Antibody (OTI1F3) - Azide and BSA Free

  • EC 3.1
  • FEN1
  • FEN-1
  • FEN-1hFEN-1
  • flap structure-specific endonuclease 1DNase IV
  • Maturation factor 1
  • maturation factor-1
  • MF1flap endonuclease 1
  • RAD2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NB100-116
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
NBP2-27500
Species: Mu
Applications: KO, PEP-ELISA, WB
NBP2-38600
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-119
Species: Hu, Mu
Applications: ICC/IF, IHC-P, IP, In vitro, RIA, WB
NBP2-16391
Species: Hu, Mu
Applications: ICC/IF, WB
NB100-74611
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-01020
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-85006
Species: Hu
Applications: IHC, IHC-P
H00005810-M01J
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, ELISA(Cap), S-ELISA, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB500-704
Species: ChHa, Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-471
Species: Hu
Applications: ChIP, ICC/IF, IHC, IP, Simple Western, WB
NB100-477
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NBP1-89929
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-148
Species: Ch, Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, PLA, WB
NBP2-70716
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for FEN-1 Antibody (NBP2-70716) (0)

There are no publications for FEN-1 Antibody (NBP2-70716).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FEN-1 Antibody (NBP2-70716) (0)

There are no reviews for FEN-1 Antibody (NBP2-70716). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FEN-1 Antibody (NBP2-70716) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional FEN-1 Products

Research Areas for FEN-1 Antibody (NBP2-70716)

Find related products by research area.

Blogs on FEN-1

There are no specific blogs for FEN-1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FEN-1 Antibody (OTI1F3) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol FEN1