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Fatty Acid Synthase/FASN Antibody

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Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).
Independent Antibodies: Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).
Orthogonal Strategies: Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq ...read more
Immunohistochemistry: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

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Fatty Acid Synthase/FASN Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDLLSCMGLRPDGIVG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FASN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Fatty Acid Synthase/FASN Recombinant Protein Antigen (NBP2-49213PEP)

Reactivity Notes

Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fatty Acid Synthase/FASN Antibody

  • EC 2.3.1
  • EC 2.3.1.85
  • EC 6.3.3.1
  • FASN
  • FASshort chain dehydrogenase/reductase family 27X, member 1
  • Fatty Acid Synthase
  • MGC14367
  • MGC15706
  • OA-519
  • SDR27X1

Background

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Fatty Acid
Synthase/FASN Antibod
NBP2-49213
Fatty Acid Synthase/FASN Antibody
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol FASN