Fatty Acid Synthase/FASN Antibodies
We offer Fatty Acid Synthase/FASN Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Fatty Acid Synthase/FASN Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Fatty Acid Synthase/FASN Antibodies can be used in a variety of model species: Chicken, Hamster, Human, Mouse, Plant, Porcine, Primate, Rat. Use the list below to choose the Fatty Acid Synthase/FASN Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Fatty Acid Synthase/FASN polyclonal antibodies and browse our Fatty Acid Synthase/FASN monoclonal antibody catalog.
Alternate Names for Fatty Acid Synthase/FASN Antibodies
anti-Fatty Acid Synthase/FASN antibody, anti-FASN antibody, anti-EC 2.3.1 antibody, anti-EC 2.3.1.85 antibody, anti-EC 6.3.3.1 antibody, anti-FASshort chain dehydrogenase/reductase family 27X, member 1 antibody, anti-fatty acid synthase antibody, anti-MGC14367 antibody, anti-MGC15706 antibody, anti-OA-519 antibody, anti-SDR27X1 antibody
11 Results for "fatty-acid-synthase-fasn" in Primary Antibodies
![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NB400-114-img0011.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.")
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NB400-114-img0010.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NB400-114-img0009.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NB400-114-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NB400-114-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Knockdown-Validated-NB400-114-img0013.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-114_rabbit-polyclonal-fatty-acid-synthase-fasn-antibody-31020241618926.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3.Lane 1: FASN transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Western-Blot-H00002194-M01-img0018.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3.Lane 1: FASN transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Immunocytochemistry-Immunofluorescence-H00002194-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Immunohistochemistry-Paraffin-H00002194-M01-img0015.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - FASN monoclonal antibody (M01), clone 3F2-1F3 Analysis of FASN expression in 293.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Western-Blot-H00002194-M01-img0017.jpg)
![Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FASN MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Immunoprecipitation-H00002194-M01-img0016.jpg)
![Sandwich ELISA Capture: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Detection limit for recombinant GST tagged Fatty Acid Synthase/FASN is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-3F2-1F3-Sandwich-ELISA-Capture-H00002194-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP1-84733-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis using Anti-FASN antibody NBP1-84733 (A) shows similar pattern to independent antibody NBP2-49213 (B).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NBP1-84733-img0011.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NBP1-84733-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84733-img0004.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP1-84733-img0006.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP1-84733-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at lipid droplet by immunofluorescent analysis. Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249415985.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at lipid droplet by immunofluorescent analysis. Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat breast. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249362797.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249342798.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249432814.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249374931.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25462_rabbit-fatty-acid-synthase-fasn-mab-hl2160-azide-bsa-free-2120249415976.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of extracts of various cell lines, using Fatty Acid Synthase/FASN antibody (NBP3-15634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Western-Blot-NBP3-15634-img0004.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of extracts of various cell lines, using Fatty Acid Synthase/FASN antibody (NBP3-15634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of NIH-3T3 cells using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunocytochemistry-Immunofluorescence-NBP3-15634-img0003.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Human colon carcinoma using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunohistochemistry-Paraffin-NBP3-15634-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of HeLa cells using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunocytochemistry-Immunofluorescence-NBP3-15634-img0002.jpg)
![Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of 300ug extracts of HeLa cells using 3ug Fatty Acid Synthase/FASN antibody (NBP3-15634). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase/FASN antibody (NBP3-15634) at a dilition of 1:500.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunoprecipitation-NBP3-15634-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Fatty Acid Synthase Antibody (325) detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase Antibody (325) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Immunocytochemistry-Immunofluorescence-NBP2-43822-img0005.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Fatty Acid Synthase Antibody (325) detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase Antibody (325) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0002.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325].](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0004.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase/FASN antibody [GT325] (NBP2-43822) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)](http://images.novusbio.com/fullsize/nbp2-43822_mouse-monoclonal-fatty-acid-synthase-fasn-antibody-325-910202419593552.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NBP2-49213-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0003.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-NBP2-49213-img0001.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Staining of Hela Cells with Fatty Acid Synthase/FASN Antibody (2A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26589_rabbit-fatty-acid-synthase-fasn-mab-2a5-282202412493622.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Staining of Hela Cells with Fatty Acid Synthase/FASN Antibody (2A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Overlay histogram showing A549 cells stained with Fatty Acid Synthase/FASN Antibody (2A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26589_rabbit-fatty-acid-synthase-fasn-mab-2a5-282202414183634.jpg)