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engrailed homeobox 2 Antibody

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Western Blot: engrailed homeobox 2 Antibody [NBP2-14947] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: engrailed homeobox 2 Antibody [NBP2-14947] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:10000.
Immunoprecipitation: engrailed homeobox 2 Antibody [NBP2-14947] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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engrailed homeobox 2 Antibody Summary

Immunogen
Full length human engrailed homeobox 2 Recombinant protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EN2
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (84%), Rat (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for engrailed homeobox 2 Antibody

  • AUTS10
  • EN2
  • engrailed homeobox 2
  • engrailed homolog 2
  • Engrailed2
  • Engrailed-2
  • Homeobox protein en-2
  • homeobox protein engrailed-2
  • hu-En-2

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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engrailed homeobox
2 Antibody
NBP2-14947
engrailed homeobox 2 Antibody
Species: Hu
Applications: WB, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol EN2
Uniprot