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EN1/Engrailed 1 Antibody (3H2)

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Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Analysis of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml
Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Analysis of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml
ELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Detection limit for recombinant GST tagged EN1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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EN1/Engrailed 1 Antibody (3H2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE*
Specificity
EN1 (3H2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EN1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.
Publications
Read Publication using H00002019-M04.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EN1/Engrailed 1 Antibody (3H2)

  • engrailed homeobox 1
  • engrailed homolog 1
  • Homeobox protein en-1
  • homeobox protein engrailed-1
  • hu-En-1

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EN1/Engrailed 1
Antibody (3H2)
H00002019-M04
EN1/Engrailed 1 Antibody (3H2)
Species: Hu, Mu
Applications: WB, ELISA, IHC, IP

Publications for EN1/Engrailed 1 Antibody (H00002019-M04)(1)


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Bioinformatics

Gene Symbol EN1
Entrez
OMIM
Uniprot