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Reelin Antibody [Unconjugated]

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Reelin was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Reelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3820) at 1.7 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Reelin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Reelin
Ala27-Met862
Accession # Q60841
Specificity
Detects mouse Reelin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RELN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3820 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reelin Antibody [Unconjugated]

  • EC 3.4.21
  • EC 3.4.21.-
  • Reeler
  • Reelin
  • RELN
  • RL
  • RLPRO1598

Background

Reelin is a 400 kDa, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The N-terminal domain binds integrin alpha 3/ beta 1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration. Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Reelin Antibody (AF3820)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
ICC
(3)
IHC
(9)
IHC-P
(1)
Immunohistochemistry
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(13)
Chicken
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF3820 Applications Species
Luckmann, MR;Nazari, EM; Cellular responses to developmental exposure to pyriproxyfen in chicken model: Contrasting embryos with and without exencephaly Neurotoxicology and teratology 2024-09-20 [PMID: 39307295] (Immunohistochemistry, Chicken) Immunohistochemistry Chicken
Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C; Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis Cancers 2024-01-19 [PMID: 38275881] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Yang, Y;Yang, R;Kang, B;Qian, S;He, X;Zhang, X; Single-cell long-read sequencing in human cerebral organoids uncovers cell-type-specific and autism-associated exons Cell reports 2023-10-26 [PMID: 37889749] (ICC, Human) ICC Human
Kira Engeroff, Davide Warm, Stefan Bittner, Oriane Blanquie Different activity patterns control various stages of Reelin synthesis in the developing neocortex Cerebral Cortex 7/24/2023 [PMID: 37288494]
N Ramezanido, DE Ouardi, M Le, S Moriceau, M Ahmadi, D Elena, D Rolland, P Bun, G Le Pen, G Canaud, N Bahi-Buiss, N Rouach, R Piskorowsk, A Pierani, P Billuart Activation of the PI3K/AKT/mTOR Pathway in Cajal-Retzius Cells Leads to Their Survival and Increases Susceptibility to Kainate-Induced Seizures International Journal of Molecular Sciences, 2023-03-11;24(6):. 3/11/2023 [PMID: 36982451]
M Riva, S Moriceau, A Morabito, E Dossi, C Sanchez-Be, P Azzam, A Navas-Oliv, B Gal, F Dori, E Cid, F Ledonne, S David, F Trovero, M Bartolomuc, E Coppola, N Rebola, A Depaulis, N Rouach, LM de la Prid, F Oury, A Pierani Aberrant survival of hippocampal Cajal-Retzius cells leads to memory deficits, gamma rhythmopathies and susceptibility to seizures in adult mice Nature Communications, 2023-03-18;14(1):1531. 2023-03-18 [PMID: 36934089] (IHC, Mouse) IHC Mouse
S Venkataram, F Saaber, P Abe, D Schütz, PA Kumar, R Stumm Cxcr4 and Ackr3 regulate allocation of caudal ganglionic eminence-derived interneurons to superficial cortical layers Cell Reports, 2022-08-02;40(5):111157. 2022-08-02 [PMID: 35926459] (IHC, Mouse) IHC Mouse
RE Niec, T Chu, M Schernthan, S Gur-Cohen, L Hidalgo, HA Pasolli, KA Luckett, Z Wang, SR Bhalla, F Cambuli, RP Kataru, K Ganesh, BJ Mehrara, D Pe'er, E Fuchs Lymphatics act as a signaling hub to regulate intestinal stem cell activity Cell Stem Cell, 2022-06-20;29(7):1067-1082.e18. 2022-06-20 [PMID: 35728595] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation Cell Stem Cell, 2021-08-02;0(0):. 2021-08-02 [PMID: 34343491] (IHC, Mouse) IHC Mouse
A Rattner, CE Terrillion, C Jou, T Kleven, SF Hu, J Williams, Z Hou, M Aggarwal, S Mori, G Shin, LA Goff, MP Witter, M Pletnikov, AA Fenton, J Nathans Developmental, cellular, and behavioral phenotypes in a mouse model of congenital hypoplasia of the dentate gyrus Elife, 2020-10-21;9(0):. 2020-10-21 [PMID: 33084572] (IHC, Mouse) IHC Mouse
Show All 23 Publications.

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Bioinformatics

Gene Symbol RELN
Uniprot