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GBX2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Placenta at dilution of 5ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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GBX2 Antibody Summary

Immunogen
Peptide with sequence C-QGKDESKVEDDPK corresponding to internal region according to NP_001476.2.
Epitope
QGKDESKVEDDPK
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GBX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:18000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
IHC-P: Human placenta shows nuclear staining of selected trophoblasts.
IHC: In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. WB reactivity reported in (PMID: 23144817).
Control
Jurkat Whole Cell Lysate
Publications
Read Publications using
NB100-55393 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23144817).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GBX2 Antibody

  • Gastrulation and brain-specific homeobox protein 2
  • gastrulation brain homeo box 2
  • gastrulation brain homeobox 2
  • GBX2
  • homeobox protein GBX-2

Background

GBX2 (Gastrulation brain homeobox 2) is a homeobox protein which is involved in enbryonic cell pluripotency and differentiation. GBX2 is expressed in all three embryonic germ layers of the late gastrula/early neurula. Gbx2 is overexpressed in a panel of human prostatic cancer cell lines (TSU-pr1, PC3, DU145, LNCaP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, ELISA, ICC/IF, IHC

Publications for GBX2 Antibody (NB100-55393)(3)

Reviews for GBX2 Antibody (NB100-55393) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GBX2 Antibody (NB100-55393) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol GBX2
Entrez
Uniprot